(4 °C). Aliquots of plasma were then immediately transferred and frozen at −80 °C until further analysis. Plasma samples were assayed for concentrations of IL-6 using a human IL-6 high sensitivity ELISA kit (R & D Systems; Minneapolis, MN, USA). The detection range for this assay ...
(7) Finally, at day 11, immunostaining treatment is conducted by using Mouse Anti-βⅢ-tubulin antibody (R&D Systems, Minneapolis, MN, USA) with fixation of the cells. The cells are fixed by 4% paraformaldehyde phosphate-buffered solution (Wako Pure Chemical Industries, Ltd., Osaka, Japan) ...