The cells with the lowest density were recognized as monocytes, using phagocytosis and size distribution analysis as criteria. Since the density of monocytes continuously increased during the centrifugation, optimal separation of monocytes and lymphocytes could only be achieved by limiting the time of ...
BACKGROUND: NK cells have been long time considered as cytotoxic lymphocytes competent in killing virus-infected cells and tumors. However, NK cells may al... G Massimo,GM Julien,N Simone,... - 《Plos One》 被引量: 87发表: 2008年 Functional characterization of CD4+CD25+ regulatory T cells...
Guillain–Barré syndrome (GBS) is an autoimmune disorder wherein the composition and gene expression patterns of peripheral blood immune cells change significantly. It is triggered by antigens with similar epitopes to Schwann cells that stimulate a mala
Lymphocyte preparations contained less than 1% monocytes and no polymorphonuclear leukocytes (PMNL), and the monocyte preparations contained less than 10% lymphocytes and no PMNL, as judged by fluorescence-activated cell sorting (FACS) forward and side scatter and CD14 immunostaining. Monocytes also ...
Immune cells, including microglia and peripherally infiltrated neutrophils, are involved in brain injury post-SAH, and their inhibition alleviates brain injury [8, 9]. Peripheral monocytes and lymphocytes also infiltrate brain tissue after SAH [10], although whether other immune cell types are ...
Monocytes washed by PBS before coculture with T lymphocytes. Luciferase assay To generate DNA promoter-activated luciferase reporter, − 2000 to -350 bp of CD274 promoter (the transcription start site is represented as + 1) as well as NFKB1 3′ UTR (as negative control) and NF...
Blood glucose is a key regulator of T lymphocytes and neutrophils [71,72]. Since an elevated neutrophil/lymphocyte ratio is widely recognized as a predictor of adverse events in ATAAD [73], intraoperative hyperglycemia may influence the pro-inflammatory activity and recruitment of monocytes through ...
and evade phagocytosis enables the bacteria to prevent clearance from the bloodstream and contribute to processes that can ultimately lead to sepsis, as reviewed elsewhere8. In some severe cases, the bacteria can reach the blood–brain barrier and infect the central nervous system, as reviewed ...
Infections are most frequent at the extremes of life, especially among newborns, reflecting age-specific differences in immunity. Monocytes maintain tissue-homeostasis and defence-readiness by escaping circulation in the absence of inflammation to become
(A) RNA was extracted from normal peripheral blood mononuclear cells (composed mainly of monocytes and lymphocytes, lanes 1 and 2) and an erythroid culture derived from normal bone marrow (lane 4) and subjected to RT-PCR analyses. PlGF primers used spanned 2 exons, giving a larger intron-...