Monoclonal antibodies are of great use in research and clinic. The first monoclonal antibody production method, hybridoma technology, was marked in the paper “Continuous cultures of fused cells secreting antibody of predefined specificity” by Milstein and Köhler in 1975 inNature. Based on that pa...
While the present invention can particularly be applied to the production of monoclonal antibodies through the production of hybridomas, other techniques directed to the immortalization of B cells can be used to generate monoclonal antibodies. For example, the use of Epstein-Barr virus to immortalize ...
monoclonal antibodies, and for workers in many disciplines who are contemplating their use. It provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology. It gives detailed and critical accounts of the theory, production, ...
This chapter provides an overview of the production of monoclonal antibodies. All the currently available mouse myeloma cell lines used for hybridoma production are of BALB/c origin, and unless there are compelling reasons to the contrary, BALB/c mice are used as recipients for the initial ...
Through Fab or Fc engineering, IgG-FcRn interaction can be used to generate a variety of therapeutic antibodies with significantly enhanced half-life or ability to remove unwanted antigen from circulation. Glycosylation of a mAb or Fc-fusion protein can have a significant impact on the PK of ...
首页ꄲFibercell中空纤维系统ꄲ单抗生产(Monoclonal Antibodies) 杂交瘤细胞会分泌转化生长因子-β(TGF-β),它是一种会抑制杂交瘤细胞生长的细胞因子。功能型TGF-β的分子量大约是 27kD,截留分子量大的培养筒(Cartridge)允许TGF- β扩散通过,所以由于浓度差异,TGF-β会自然进入循环培养基,可以减小或者消除对细胞的...
Therapeutic monoclonal antibodies (mAbs) have become the cornerstone in the clinical practice of modern biotherapeutics, especially in oncology, inflammation and autoimmune diseases. They are the most dominant class of biologics in current drug development pipelines. The success of mAb as therapeutics in...
The monoclonal antibodies do not show any cross-reaction with other commonly used microbial enzymes, and they allow the detection of ANX in complex mixtures by ELISA inhibition assays down to the concentration limit of approximately 10 g kg1. These mAbs are a valuable tool to detect and ...
Separation and determination of remnant-like particles in human serum using monoclonal antibodies to apo B100 and apo AI. Remnants of triglyceride-rich lipoproteins have been considered to be atherogenic. In practice, remnant levels have usually been estimated as intermediate density lipoproteins (IDL) ...
The anti-RAI3 monoclonal antibodies bound to recombinant human RAI3 protein with high specificity and affinity, as shown by ELISA, western blot and ICC. The cDNA dot blot and immunohistochemical experiments showed that both RAI3 mRNA and RAI3 protein were abundantly expressed in human breast carci...