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gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins. ...
Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complement...
Monoclonal antibodiesMethods and Protocolsdoi:10.1007/978-1-62703-992-5X R.GeHumana PressSheng li ke xue jin zhan Progress in physiology
[Methods in Molecular Biology] Antibody Engineering Volume 1827 (Methods and Protocols) || Fc Engineering: Tailored Synthetic Human IgG1-Fc Repertoire for ... The therapeutic development of monoclonal antibodies requires robust and reliable methods for their recombinant expression and characterization. In...
Monoclonal antibodies (mAbs) are used to prevent, detect, and treat a broad spectrum of non-communicable and communicable diseases. Over the past few years
Recently, a role of AMPylation as a universal regulatory mechanism in infection and cellular homeostasis has emerged, driving the demand for universal tools to study this modification. Here, we describe three monoclonal anti-AMP antibodies (mAbs) from mouse that are capable of protein backbone-...
The immunoassay multiplexing capacity relies on monoclonal antibodies labeled with stable heavy-metal isotopes. To date, a variety of rare-earth elements and noble and post-transition metal isotopes have been used in mass cytometry; nevertheless, the methods used for antibody conjugation differ because...
2. Materials and methods 2.1. Materials mAb1, mAb2, mAb4, and mAb5 are IgG1 monoclonal antibodies, mAb3 is an IgG1 Fab antibody without Fc region, and mAb6 is an IgG4 monoclonal antibody. All antibodies are developed in Genentech, Inc. C-terminal AviTag FcRn was prepared as described...