The captured fusion protein was cleaved by PreScission protease (Amersham Pharmacia Biotech) at a ratio of 1% (wt/wt) at 4°C and HsPex13p-SH3(wild-type or I326T) protein was eluted by buffer-A. Protease susceptibility assay. Twenty microliters of GST-Pex13p(wild- type) or (I326T)...
The spirocyclic part consisting of an α-acylated tetronic acid and a multisubstituted cyclohexene embedded in versipelostatin, a novel GRP78/Bip molecular chaperone downregulator, has been synthesized in enantiomerically pure form. The asymmetric synthesis of the targeted spiro[4.5]-1-oxa-7-decen-...
and dried with Na2SO4. After filtration, the Molecules 2021, 26, 482 9 of 15 filtrate was evaporated to afford the crude product as a solid, which was then washed with diethyl ether to give anilide 3 (0.651 g, 34% yield). N-(Boc)-L-phenylalanine p-nitroanilide (3): 1H NMR (...
The dosage of the lignin polymer required for reaching the maximum removal had a linear relationship with the dye concentration. The presence of inorganic salts including NaCl, NaNO3, and Na2SO4 had a marginal effect on the dye removal. Under the optimized conditions, greater than 98% of RB5...
The organic layer was washed with brine, dried over anhydrous Na2SO4, filtered, and the solvent was removed in vacuo. The residue was purified by silica gel column chromatography (petroleum ether: ethyl acetate = 2:1) to afford compounds 5a–5c, the NMR spectrums of compounds 5a–5c were...
Indeed, the stable phase in contact with a saturated solution of Na2SO4 is mirabilite (Na2SO4·10H2O) or thenardite (anhydrous Na2SO4) depending on whether the temperature is below or above 32.4 ◦C, respectively. The heptahydrate (Na2SO4·7H2O) may also exist below 32.4 ◦C, ...
2. Materials and Methods 2.1. Samples Preparation 2.1.1. Ti25Nb10Zr Alloy Preparation and Preliminary Characterization Ti25Nb10Zr (in wt %) was manufactured by Romanian Company (R&D Consulting and Services, Bucharest, Romania). Ti25Nb10Zr alloy was casted by a cold crucible levitation melting...
Cooper and co-workers reported the syntheses of homochiral alkylated organic cages functionalised with twelve n-hexyl, n-pentyl, isohexyl or n-octyl tails by a one-step [6+4] condensation of the corresponding (S,S)-1,2-functionalised-1,2-diamines with 1,3,5-triformylbenzene in ...