In vivo experiments confirmed the activation of reactive astrocytes and microglial cells in TBI and importantly demonstrated that Wtap knockdown improved neuroinflammation and functional impairment. These findings underscore the significance of Wtap/Ythdf1-mediated Lcn2 regulation in TBI secondary injury and...
Despite the known impact of YTHDF1 on tumor cells, its role in the tumor microenvironment (TME) is less well-documented. Immunotherapy has advanced in treating cancer, yet solid tumors respond at a rate below 30%, largely due to the immunosuppressive nature of the TME [9, 10]. Tumor-...
研究发现肝细胞中可被OXA上调的YTHDF2在阻碍肝癌进展中发挥了关键作用。肝脏YTHDF2对于维持肝脏的抗肿瘤反应至关重要。在小鼠模型和HCC患者中,肝细胞中YTHDF2的缺失降低了CX3CL1 mRNA的稳定性,损害了CD8+T细胞功能,促进了肿瘤生长。OXA...
resistant and 19 sensitive samples (Fig.S1A, B). By analyzing the mRNA-seq data, we observed down-regulation of two readers (ALYREFandYTHDF1), one writer (NSUN5), and one eraser (ALKBH1) in radioresistant samples compared with sensitive samples (Fig.1B and Fig.S1C, D). The mRNA ...
In addition, single-cell RNA sequencing identified changes in cell type abundance and distribution in C9-ALS/FTD organoids, including a reduction in the number of deep layer cortical neurons and the distribution of neural progenitors. Further, molecular and cellular analyses and patch-clamp ...
Additionally, among the hundreds of screened natural secondary metabolites, alkaloids embody the largest group of low molecular weight organic nitrogenous compounds isolated from terrestrial and marine sources [14]. The structural diversity of this family is actually due to the wide...
Major depressive disorder (MDD) is a chronic, generally episodic and debilitating disease that affects an estimated 300 million people worldwide, but its pathogenesis is poorly understood. The heritability estimate of MDD is 30–40%, suggesting that gene
The active metabolite of 5-Fluorouracil (5FU), used in the treatment of several types of cancer, acts by inhibiting the thymidylate synthase encoded by the TYMS gene, which catalyzes the rate-limiting step in DNA replication. The major failure of 5FU-bas
E Inhibition of the ITGB1 expression in glial cells may lead to neurodegenerative and behavioral abnormalities in adult mice. F YTHDF2 induces instability of ITGB1 mRNA and leads to mRNA degradation in the form of m6A. After ITGB1 silencing, it inhibits the FAK/PI3K/AKT signaling pathway. ...
Recently, m6A modulators, including “writers” (METTL3, METTL14, WTAP and RBM15), “easers” (ALKBH5 and FTO), and “readers” (YTHDF1/2/3, HuR and HNRNPA2B1), have been considered to be essential for regulation of cancer biology, thus, m6A modification in cancers is gradually ...