It is possible to tailor the dynamic response of polyborosiloxanes(PBS)based on picking the right molecular weight of a precursor.The molecular weight of hydroxy-terminated polydimethylsiloxane(PDMS)defines the shear-stiffening performance of PBS due to straightforward condensation reaction between hydroxy...
Cells were extracted with PBS containing 0.3% Triton-X for 5 min and fixed in 4% paraformaldehyde for 15 min. The coverslips were washed three times with PBS containing 0.1% Triton-X before being incubated with antibodies at a 1:1000 dilution in PBS containing 3% BSA and 0.1% Triton-...
The cells were washed with PBS and resuspended in MHS buffer (20 mM HEPES and 225 mM sucrose, pH adjusted to 7.4 with MgO) at a concentration of 1.5 × 107 cells/mL. 10 μL cells were taken out and used for protein expression detection. Next, 100 μL cells were mixed with ...
6)weight average molecular weight重均相对分子质量 1.The hydrolytic properties of linear and 1 ,2,4-butanetriol-grafted polybutylene succinate (PBS) were studied based on the change in weight average molecular weight (Mw) , melting point and surface morphology.从重均相对分子质量(Mw)、熔点以及表面...
For the preparation of neutrophils, blood was separated using Ficoll solution and the pellet was thoroughly resuspended in 50 ml 1 % Dextran/PBS. The solution was incubated 30 min at RT and the supernatant harvested carefully and centrifuged 10 min at 500 x g at 4 °C. In order to lyse ...
(2013). The exponential-growth cells were subjected to the stress conditions for 3 h suspended in either rich medium of PBS buffer, followed by determination of the cells’ survival, the activity of the main ROS scavenging enzymes, and malondialdehyde content, which is a marker of lipid ...
Antibacterial coating of medical devices is a promising approach to reduce the risk of infection but has not yet been achieved on wear surfaces, e.g. polyethylene (PE). We quantitatively determined the antimicrobial potency of different PE surfaces, which had been conversed to diamond-like carbon...
The shape of the easily degradable plastics (PCL and PBSA) was found to affect biodegradation only at the beginning of the process, while the slowly degrading plastics (PLLA and PBS) throughout the biodegradation. At the early stage of the biodegradation, the biodegradable plastics having a ...
Immunofluorescence Co-localization of TH/α-syn Sections were incubated with the following primary antibodies: polyclonal anti-TH (1:250; Merck-Millipore) and monoclonal anti-α-syn (1:50; Santa Cruz Biotechnology) as previously described [51]. Sections were washed with PBS and were incubated wit...
Cells were collected by centrifugation at RT at 300 g for 5 min, washed with 10 mL of PBS, and re-suspended with 0.5 mL PBS. The resuspension was applied to a BD FACSAria Fusion machine operated under software BD FACS Diva (version 8.0.3) for FACS. The top 5% green ...