Mixtures of bovine serum albumin (BSA) and several low molecular weight phenolics were incubated and fractionated using G-50 Sephadex chromatography. Fractions corresponding to the protein and the possible phenolic-protein complexes and fractions corresponding to the free phenolics were collected, and ...
Each band is labeled with its molecular weight and protein name. * MW = molecular weight. 1. from rabbit muscle; 2. from Escherichia coli; 3. from human serum; 4. BSA = bovine serum albumin; 5. from bovine liver; 6. from chicken egg; 7. from bovine erythrocyte; 8. from soy- ...
Concentrations of proteins and polysaccharides were usually determined by Lowry method and phenol-sulfuric acid method with BSA and glucose as for standard substances. On one hand, these standards might not be appropriate to represent the proteins and polysaccharides in reclaimed water26,27. On the ...
the protein sample is incubated with an enzyme that is able to remove parts or full glycan chains. WB protein species from the digested sample are then compared with the undigested sample, and any observed shift in molecular weight indicates...
The predicted molecular weight (MW) of the protein is the sum of the molecular weights of all the protein's amino acids. It is easy to calculate, e.g., using the freeonline ExPASy tool.Often the calculated MW is different from that observ...
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Humic substances (HS) are complex mixtures of natural organic material which are found almost everywhere in the environment, and particularly in soils, sediments, and natural water. HS play key roles in many processes of paramount importance, such as pla
Briefly, total protein concentration was determined using the bicinchoninic acid (BCA) method with bovine serum albumin (BSA) as a standard (Pierce). Equivalent amounts of protein from quadriceps, triceps, and soleus muscles were subjected to 8–15% SDS-PAGE and semi-dry transferred to PVDF ...
Optimal secondary structure prediction of the query protein domains was ensured by the DSC method36 within SeqFold. The engrailed homeodomain (PDB code: 1DU0) was identified as the best structural template of the NF-HEV amino-terminal domain (NF-HEV aa 1–65). We used the threading-derived...
Chantret N, Sourdille P, Röder MS, Tavaud M, Bernard M, Doussinault G (2000) Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat. Theor Appl Genet 100:1217–1224 Article CAS...