Journal of Food ScienceBartolome B., Hernandez T., Estrella I., Interactions of low molecular weight phenolic with proteins (BSA). J. Food Sci., 2000, 65, 617-621.Bartolome, B., Estrella, I., Hernandez, M. T. 2000. Interaction of Low Molecular Weight Phenolics with Proteins (BSA). ...
Blocking buffers (e.g., 5% skimmed milk, casein, or BSA) Incubation and washing times (e.g., overnight at 4C or 1.5h at RT) Secondary antibodies used for detection (e.g., dilution factor) Membranes types (nitrocellulose vs. PVDF). ...
After being cooled to room temperature, the sections were washed with phosphate buffer saline (PBS), blocked with 5% bovine serum albumin (BSA) for 30 min at 37 °C, and then incubated overnight at 4 °C with rabbit anti-mouse KCNMA1 primary antibodies (1:100, CSB-EP012077PI, ...
Probe for a protein which is known to be of high abundance in your sample e.g. a house keeping protein Buffers may be incompatible with antibody or method of detection Check what buffers the manufacturer recommends e.g. milk blocking or BSA ...
For protein detection, membranes were incubated with primary antibodies (diluted in TBS-T/1 % BSA/N3) overnight at 4 °C or for 1 h at RT. The next day, membranes were washed 3 times with TBS-T and incubated for 1 h with a horseradish peroxidase (HRP)-conjugated secondary antibody ...
aHowever, the BSA affinity of high molecular weight fraction was higher than low molecular weight fraction with higher content of phenolic hydroxyl group (Figure 6) 然而,高分子重分数的BSA亲合力低分子量的分数高于与更高的内容酚醛羟基(图6)[translate]...
The intermolecular interactions of cholinium-based ionic liquids (ILs) with biological origin anions (amino and dicarboxylic acids) with bovine serum albumin (BSA) and human serum albumin (HSA) have been studied by spectral and computational methods. The choline malonate ([Ch][Mal]), choline suc...
The fluorescence data also indicated 1:1 binding stoichiometry of the complex on one binding site of BSA. Competitive experiments with site markers (phenylbutazone and ibuprofen) and synchronous measurements identified site I of BSA as the main binding site. Molecular docking results confirmed the ...
Agrobiodiversity is the variety and variability of animals, plants and microorganisms that are used directly or indirectly for food and agriculture, including crops, livestock, forestry and fisheries. It also includes the diversity of non-harvested speci
The coverslips were washed three times with PBS containing 0.1% Triton-X before being incubated with antibodies at a 1:1000 dilution in PBS containing 3% BSA and 0.1% Triton-X overnight at 4 °C. Secondary antibody incubations were performed by incubation at room temperature for 1 h ...