To date, a few leading technologies such as traction force microscopy, optical/magnetic tweezers, and molecular tension鈥揵ased fluorescence microscopy are broadly used in measuring cellular forces. However, the considerable limitations, regarding the sensitivity and ambiguities in data interpretation, are...
Images of stained samples were captured by confocal fluorescence microscopy (Zeiss, LSM780, Germany). Fluorescent image analysis All images were analyzed by ImageJ (NIH, U.S.A.). For collagen Ι quantification, the mean intensity of four random fields across three samples per group was measured...
(Leake, 2013), especially when used for emerging correlative microscopy approaches that allow multiple orthogonal data types to be acquired (Leake, 2021) including potential molecular orientational information from super-resolved fluorescence polarization microscopy (Shepherd, Payne-Dwyer, Lee, Syeda, & ...
Component analysis of MW > 10 kDa component were carried out by three-dimensional excitation-emission matrix fluorescence spectra (EEM), attenuated total reflection fourier transformed infrared spectroscopy (ATR-FTIR) and confocal laser scanning microscopy (CLSM), revealing its major foulant ...
Total internal reflection fluorescence microscopy was used to follow total filament length as a function of time. To illustrate filament growth, a series of TIRF images are shown as a function of time (Fig. 2A). At the light microscope level, R179H polymerizes into filaments that are ...
Fluorescence microscopy FSHD: Facioscapulohumeral muscular dystrophy GMC: Genomic Morse code HCM-FISH: Heterochromatin-oriented M-FISH MCT: Molecular combing technology MCB: Multicolor banding M-FISH: Multicolor-FISH pod-FISH: Parental origin determination FISH Q-FISH: Quantitative FISH RCA...
After incubation with the fluorescent dyes, coverslips were mounted on cover glasses using mounting medium, without performing fixation and images were immediately acquired using epifluorescence microscopy. Images were quantified and analyzed using ImageJ software, using gray scale to quantify the fluorescen...
performed on cells cultured on the gels after 2% paraformaldehyde fixation and 0.1% Triton X (Sigma) permeabilization. For experiments involving Ki67 staining, microscopy settings (exposure time, gain, etc.) remained constant to allow for qualitative comparisons in fluorescence signal across different ...
Single-molecule techniques, such as atomic force microscopy, single-molecule fluorescence microscopy and optical tweezers, have been key in resolving the mechanisms behind the power strokes, 'processive' steps and forces of cytoskeletal motors. However, it remains unclear how single force generators are...
To localize caveolae-related proteins on single caveola at the nanoscale, we developed a two-color super-resolution fluorescence microscopy (stimulated emission depletion, STED) method. First, MEF plasma membrane sheets were immuno-stained against caveolin1 (a marker for caveolae, Fig. 3a). As ill...