The GlcNAc-eluted solution was loaded onto a DEAE-Sepharose column equilibrated with buffer I and the DHPR/L-type Ca2+ channels were eluted with a step gradient of NaCl from 100 mM to 300 mM in buffer I. The fractions were analysed by SDS-PAGE and the fractions containing purified ...
Consistent with the in-gel fluorescence results, the complex structure contained five subunits with 1:1 stoichiometry. The overall GPI-T architecture assumes the shape of a canon (the luminal domain, mainly from PIGT/GPAA1/PIGS/PIGK) on a carriage (the transmembrane domain, TMD, mainly from ...
Proteins and other biomolecules function in cellular environments that contain significant concentrations of dissolved salts and even simple salts such as NaCl can significantly affect both the kinetics and thermodynamics of macromolecular interactions. As one approach to directly observing the effects of sal...
Funazukuri, Molecular dynamics simulations on ion- pair association of NaCl from ambient to supercritical water, Fluid Phase Equilibr. 297 (2010) 227-235.Yui K, Sakuma M, Funazukuri T (2010) Molecular dynamics simulation on ion-pair association of NaCl from ambient to supercritical water. Fluid...
(N,N-dimethyl-N-hexadecylammonium acetoxy)dichloride (16-E2-16) influences the micellization of promethazine hydrochloride (PMT) studied through surface tension, UV and FT-IR measurements in various media (aqueous, 50 mmol/kg sodium chloride (NaCl), 500 mmol/kg urea (U) and 500 mmol/kg ...
The aim of this study was to investigate the cytotoxic activity of the Coriandrum sativum (C. sativum) ethanolic extract (CSEE) in neuroblastoma cells, chemically characterize the compounds present in the CSEE, and predict the molecular interactions and
In Arabidopsis, CSD1 exhibited the highest expression in stem tissue but lower expression or no change in response to drought stress (e.g., ABA, NaCl, and PEG treatments) [78]. Therefore, the lower expression of CSD1 under drought conditions observed in this study might suggest the ...
Samples were subsequently washed with Tris-buffered saline (TBS) (12.5 mM Tris, 0.9% NaCl, pH7.6), blocked with PBS, 0.3% bovine serum, 4% normal serum (according to the species in which the secondary antibody was developed) and incubated overnight in specific primary antibodies at 4°C....
The pellet was twice resuspended in 10 ml Triton-X buffer (0.25% Triton X-100, 10 mM EDTA, 10 mM Tris/HCl (pH8.1), 10 mM NaCl, 1× CompleteMini protease inhibitor (Roche)), shaked on ice for 10 min and centrifuged at 2000 rpm, 4°C. The nuclear lysate was resuspended in SDS ...
Four-point calibration solutions were prepared by dissolving serotonin (Sigma-Aldrich) into Tris buffer to produce solutions with concentrations of 10, 25, 50, and 100 nM. Tris Buffer consisted of 15 mM Tris Buffer consisted of 15 mM H2NC(CH2OH)2 HCl, 140 mM NaCl, 3.25 mM KCl, 1.2 mM ...