We have studied (NaCl)n clusters (n=1鈥 48) on the (100) MgO surface using the atomistic simulation technique. The geometry, binding energy, and interaction... AL Shluger - 《Journal of Vacuum Science & Technology B》 被引量: 7发表: 1995年 Molecular geometry of 'ionic molecules': A ...
The surface-enhanced Raman spectrum of lawsone, a well known natural dye, has been investigated. Activation with KNO 3 or Na 2SO 4 solution was necessary to enhance the Raman signal, whereas addition of NaCl solution depletes the effects. In the enhanced Raman spectrum, the strong double-...
which can inhibit GPCRs with constitutive activity, are considered useful therapeutic agents, but the molecular mechanism of such ligands remains insufficiently understood. Here, we report a crystal structure of the ghrelin receptor bound to the ...
For hSMPD2 protein purification, two liters of transfected HEK293F cells were harvested by centrifugation at 3000 g. Cell pellets were resuspended in lysis buffer containing 25 mM Hepes, pH 7.4, and 150 mM NaCl, 1 μg/mL leupeptin, 1.5 μg/mL pepstatin, 0.84 μg/mL aprot...
The aim of this investigation is to evaluate the effects of adding Al2O3 nanoparticles with concentration of 0 and 0.05 as well as influence of geometry, fin installation, and other influential factors on these processes. The simulations were performed using ANSYS Fluent 18.1 and verification with...
Molecular dynamics simulations of charged plates and electrolyte in a bilayer-like geometry: Molecular ordering and electrostatic potential We have performed molecular dynamics (MD) simulations of NaCl solution separated by two charged plates. In order to establish its applicability to future s... JN ...
Following nickel chelation chromatography purification of the lysate, the protein was loaded on to a Superdex S75 26/60 (Amersham) gel filtration column that was equilibrated with 20 mM Tris, pH 8.0, 150 mM NaCl. This purified material was used for all other experiments. Circular dichroism CD...
The geometry consists in two OH bonds of length 0.9572 Å with an angle θ = 104.5° between them. The OX line forms an angle θ/2 with the OH bonds. Each H site has an effective charge of 0.52e, and these charges are neutralized by the negative X charge. The oxygens of the wa...
Similar distances to the transition state were observed for either the WT-RBD or the VoCs-RBD interacting with the ACE2 receptor, indicating a similar binding geometry/location of the binding pocket under these conditions. However, a decrease in the dissociation rate extrapolated to zero force was...
For protein purification, the cells were first dispersed by a hand-held homogenizer in lysis buffer (50 mM HEPES pH 7.4, 300 mM NaCl, and 15% glycerol) supplemented with protease inhibitors (1 μg/mL aprotinin, 1 μg/mL leupeptin, 1 μg/mL pepstatin, 20 μg/mL trypsin inhibitor,...