For the lactate assay (Abcam). 2 × 106cells per assay were processed in ice-cold lactate assay buffer, centrifuged to collect the supernatant, and deproteinized using the TCA kit (Abcam). After adding the reaction mix, the samples incubated at room temperature for 30 min and lactate ...
For that, we carried out metabolomic profiling using 1H-NMR spectroscopy and found marked differences in the levels of lactate, acetate, and succinate metabolites. Altered metabolite levels have also been observed in several other neurological disorders such as schizophrenia, down syndrome, and ...
Aberrant aerobic glycolysis in tumor cells leads to lactate accumulation and TME acidification, hindering immune cell activity [49, 50]. Understanding immune cell metabolism holds potential for metabolism-targeted therapies, enhancing immunotherapeutic efficacy. Previous studies have demonstrated that T cell ...
Actively replicating cancer cells are faced with a lack of oxygen (hypoxia) and thus have to switch their energetic balance from oxidative phosphorylation to glycolysis. Glycolysis, which is based on anaerobic conversion of glucose to lactate, becomes the major source of ATP and NADH in the cells...
Pyroptosis was quantified using lactate dehydrogenase (LDH) release, and the caspase-1 inhibitor VX-765 was used to selectively assess the role for inflammasome activation in cell death. We observed that nigericin treatment of S-clamp primed MDMi readily triggered caspase-1-dependent pyroptosis within...
Optimized dendritic cell-based immunotherapy for melanoma: the TriMix-formula. Cancer Immunol Immunother. 2014;63:959–67. Article PubMed CAS Google Scholar Rosenberg SA, Restifo NP. Adoptive cell transfer as personalized immunotherapy for human cancer. Science. 2015;348:62–8. Article CAS Pub...
(Miyahara et al. 1982; Murakami et al. 1986): for example, a glucose sensor using glucose oxidase, a lactate sensor using lactate dehydrogenase, an acetylcholine sensor using acetylcholine esterase, an L-glutamate sensor using L-glutamate decarboxylase, a tributylene sensor using lipase, and an ...
A reduction of oxygen supply also leads to the accumulation of lactate via anaerobic glycolysis and so to acidosis [40–43]. Lipid peroxidation Besides the production of different species of oxygen radicals, acidosis also interferes with intracellular protein synthesis. Lipid peroxidation appears to ...
(ADCC) relies on cell-mediated immunity, where the effector cells actively lyse tumor cells which have been bound by a specific antibody. To determine the ADCC potential of our humanized antibodies, we evaluated their efficacy to kill MDA-MB-231 cells by ADCC using lactate dehydrogenase (LDH) ...
Objective: To explore the lactate-related genes (LRGs) in lung adenocarcinoma (LUAD) by various methods, construct a prognostic model, and explore the relationship between lactate subtypes and the immune tumor microenvironment (TME). Methods: 24 LRGs wer