The chemical formula of a compound is a symbolic representation of its chemical composition. Chemical formulae provide insight into the elements that constitute the molecules of a compound and also the ratio in which the atoms of these elements combine to form such molecules. For example, the ...
the hydrophilic/hydrophobic nature of the channel residues, and the spatial orientation of specific residues that provide hydrogen acceptors/donors for the water and glycerol molecules that traverse the channel. For thesemembrane channels, such simulations have been particularly instructive and serve to di...
DAVID: database for annotation, visualization, and Integrated Discovery. Genome Biol. 2003;4:P3. Article PubMed Google Scholar Eltahir HM, et al. Alpha-Mangostin ameliorates acute kidney injury via modifying levels of circulating TNF-α and IL-6 in glycerol-induced rhabdomyolysis animal model....
Cells were resuspended in Ni-Wash buffer (25 mM HEPES, 500 mM NaCl, 10% glycerol, 1 mM DTT, 10 μM DHT, 1% Tween-20, 20 mM imidazole at pH 7.4), lysed and centrifuged. Soluble protein was captured by IMAC and eluted with 500 mM imidazole. During an overnight ...
After 18 h of post-induction incubation, cells were harvested by centrifugation at 3300 × g for 15 min and suspended in lysis buffer, containing 50 mM Tris-HCl (pH 8.0), 300 mM NaCl, 10 mM imidazole, and 5% glycerol. The cell suspension was sonicated for 4 min on ...
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It’s mainly asolvent for ingredientsthat do not like to dissolve in oils but rather in water. Glycerin- superstar Also-called:Glycerol|What-it-does:skin-identical ingredient,moisturizer/humectant|Irritancy:0|Comedogenicity:0 A natural moisturizer that’s also in our skin ...
For immunoprecipitation, cell lysates or animal brains were lysed in Nonidet P-40 cell lysis buffer, 0.5 M Tris pH 7.3, 5 M NaCl, 1 M MgCl2, 1 M EDTA, and 80% glycerol with protease inhibitors. Cell lysates were added to prewashed magnetic beads (70,024 S, Cell Signaling Technology) ...
We subsequently performed whole-transcriptome RNA-Seq for all experimental conditions and mass-spec proteomics for the majority of them. (No proteomics was performed for the low-magnesium experiment.) We considered four different carbon sources: glucose, glycerol, gluconate, and lactate; we also ...
Protein was extracted from PBS-washed cell pellets using RPPA lysis buffer (1% Triton X-100, 50 mmol/L HEPES (pH 7.4), 150 mmol/L NaCl, 1.5 mmol/L MgCl2, 1 mmol/L EGTA, 100 mmol/L NaF, 10 mmol/L NaPPi, 10% glycerol, 1 mmol/L phenylmethylsulfonyl fluoride, 1...