The mutagenesis identified critical residues at the active site and suggested their possible contributions. Substituting R60 at the S1 site with glutamate almost diminished GPI-T activity (9.8% relative to the wild-type (WT) and same hereafter), and R60A, R60L, or even the conservative R60K m...
The glycerol content-dependent onset temperature for this transition was 70掳C for pure starch and decreased to 40掳C for a matrix with 30% glycerol. Measurements of the rate of non-radiative decay from the Ery B triplet state indicated that glycerol plasticized the starch matrix above 10wt.%...
To confirm the self-assembling activity of the Cep63 P1•Cep152 M4d complex described above, we examined whether mCherry-Cep63 FL and mGFP-Cep152 M6(924–1295) (the longest Cep152 form that we successfully purified) can form self-assemblies under the same conditions described in Fig.2c....
Interestingly, the nsP1-m7GMP structure was obtained when we incubated nsP1 with a 12-mer RNA from the 5′ end of the CHIKV viral genome with a Cap-0 structure at the 5′ end (m7GpppAUGGCUGCGUGA, hereafter named m7GpppAU-10). This indicates that wild-type (WT) nsP1 can cleave ...
Cell wall integrity (CWI) and high osmotic glycerol (HOG) signaling pathways are two conserved MAPK pathways (Li et al.2012). An unknown plasma membrane-localized receptor, which functions upstream of the CWI pathway, senses cell-wall stresses to activate the Mck1-Mkk1-Mps1 protein kinase cas...
The influence of nonionic surfactant type and concentration on the kinetics of Ostwald ripening (OR) of hydrocarbon emulsion droplets suspended in aqueous micellar solutions was investigated. OR rates were determined by measuring changes in the droplet size distribution of 5 wt % n-tetradecane oil-in...
Ligand-free protein was produced by growth on the glycerol based medium, Enfor’s minimal media. In all cases, tmCBP was purified as previously described [13, 32], with slight modifications. Cell pellets were lysed by sonication. The resulting lysate was clarified by centrifugation (34,000 ...
THEORETICAL PREDICTION OF THE GLASS TRANSITION TEMPERATURE OF GLYCEROL-WATER BINARY CRYOPROTECTANT The glass transition temperature(T_g)of the glycerol aqueous solution(60 wt/%)was determined by isothermal-isobaric molecular dynamic simulation(NPT-MD).Th... DX Li,BC Wang,BL Liu,... - 《Journal ...
Diterpene synthase VenA is responsible for assembling venezuelaene A with a unique 5-5-6-7 tetracyclic skeleton from geranylgeranyl pyrophosphate. VenA also demonstrates substrate promiscuity by accepting geranyl pyrophosphate and farnesyl pyrophosphate
region.bCompound 2 covalently binds to C3476.36band mounts on the membrane-facing surface of the cytoplasmic end of TM6.cRepresentative mutation effects on the ago-allosterism associated with compound 2. Data are presented as means ± S.E.M. of three independent experiments. WT, wild-...