METHODS: Cells cultured in 3D collagen gels were divided into the H460 group (HG), the mononuclear cell group (MG) and the co-culture group of H460 and mononuclear cells (HMG), respectively. The growth of cells was observed under microscope. The expressions of MMPs were detected by ...
Full size image Direct effects in path analysis represented partial regression coefficients for each exogenous on the endogenous variable. A direct effect was considered to be significant if p < 0.05. The direct effects were presented in a standardized form. The modification index (MI) was also...
(C) Gelatin zymography assay reveals less active MMP-9 in cell culture supernatant of PBMCs (cultured for 72 h with TNF-α) of patient-R versus controls and FOP patients (n = 3). (D) Quantification of gelatin zymography in (C). (E) Schematic of MMP-9 protein denoting the ...
IL-1-induced expression of MMP1 and MMP13 in the human chondrocyte cell line SW1353 was suppressed by the addition of HDAC inhibitor TSA (Fig. 1A) as has been shown previously for a number of HDAC inhibitors8,9. A Zn2+-dependent class I, II and IV HDAC RNAi screen was performed in...
yb-2353R Mast Cell Chymase肥大细胞类糜蛋白酶1抗体 ybm-2059M Metronidazole(1C2)甲硝唑单克隆抗体 yb-3834R MrpL12线粒体核糖体蛋白L12抗体 yb-3835R MrpL39线粒体核糖体蛋白L39 yb-3788R Mitochondrial Pyruvate dehydrogenase kinase 1丙酮酸脱氢酶激酶1抗体 yb-4138R MAPK11丝裂原活化的蛋白激酶p38β抗体...
Form Liquid Storage buffer PBS buffer pH 7.4. Concentration 1 mg/ml Purity >95% Clonality Monoclonal Isotype IgG1, kappa Applications ELISA, ICC/IF, B/N, WB Purification Protein A or G purified from cell culture supernatant. Endotoxin level Please contact with the lab...
Membrane-type matrix metalloproteinases (MT-MMPs) have been shown to activate pro-MMP-2 on the cell surface and are suggested to be key enzymes in tissue remodelling under various physiological and pathological conditions. To investigate the role of MT-MMP in progressive renal injury, the gene ...
Two variants of the MDA-MB-231 human breast cancer cell line selected for enhanced ability to form brain metastases in nude mice (231-BR and 231-BR3 cells) were found to express high levels of matrix metalloproteinase-1 (MMP-1). Short hairpin RNA-mediated stable knockdown of MMP-1 in 23...
MMPs were previously detected in the rat Sertoli cell39 and accessory sex glands40. The mechanism to control MMPs activation is complex and includes regulation of gene expression, cleavage of latent forms, and inhibition of active MMPs by their endogenous inhibitors: TIMPs35. In this study we di...
Full size image MCP-1 produced in mIVD induced macrophage migration mIVDs were cultured in the presence or absence of 100 nM of thrombin for 72 h. The culture supernatants were collected and poured to the lower Chemotaxicell chamber. Upper wells were populated with 3 × 105 macrophag...