FAQs: MMLV-RT I want to generate cDNA from viral RNA with very high secondary structure, can I use MMLV-RT? Can I improve the yield when using MMLV-RT? What buffer do you recommend using with MMLV-RT? How is MMLV-RT inactivated? Does MMLV-RT need a primer? What is the error rate...
55C MMLV-RT GC-rich Regions Robust thermostable reverse transcriptase ideal for ambient temperature stable molecular assays. R everse transcriptase activity up to 60°C Ideal for RNA with high secondary structure such as viral genomes S ensitive detection of low copy number RNA targets ...
Directed specific mutations can promise to produce thermostable and soluble RT commercial enzymes. Predicted hot mutations in the active site of the RNase H domain of recombinant RT without any effect on its structure.doi:10.1186/s13568-024-01773-6Divbandi, Marzieh...
1. The M-MLV RT Reaction Buffer is compatible with enzymes used in a number of downstream applications. Phenol extractions and ethanol precipitations typically are not necessary before performing second-strand synthesis and amplification. 2. If there is concern about possible RNase contamination in ...
The major addition of a single +C at the cDNA–cTSO junction conveys the idea that the m7G cap structure itself may act as a template for the RT during cDNA synthesis, making the first nontemplated addition of +C, in reality, templated. It is unclear at this point whether this extra cy...