Changes in: apoptosis, cell cycle distribution, NFkB activity, protein or mRNA expression levels of Bcl-2 family members/cell cycle regulators were determined using the cell titer glo assay, flow cytometry, imagestream technology, and Western blotting, respectively. Subsequently, MCL cells were ...
Pevonedistat+TNF-αis cytotoxic. (a) Cultured rat H-4-II-E cells were treated with pevonedistat in combination with either PBS (gray boxes) or 5 ng/ml TNF-α(black triangles) for 24 h. The LC50was determined using the least-squares method, and solid lines indicate a non-line...
Katsiki N, Tziomalos K, Chatzizisis Y, Elisaf M, Hatzitolios AI . Effect of HMG-CoA reductase inhibitors on vascular cell apoptosis: beneficial or detrimental?Atherosclerosis2010;211: 9–14. ArticleCASGoogle Scholar Deuse T, Hua X, Wang D, Maegdefessel L, Heeren J, Scheja Let al. Di...
Mineralocorticoid receptor deficiency in macrophages inhibits neointimal hyperplasia and suppresses macrophage inflammation through SGK1-AP1/NF-kappaB pathways. Arterioscler Thromb Vasc Biol 2016; 36: 874–885. 54. Guo X, Shi N, Cui XB, Wang JN, Fukui Y, Chen SY. Dedicator of cytokinesis 2, a ...
结论:MLN4924能够明显抑制A549细胞增殖、促进细胞凋亡,其相关机制为MLN4924可阻滞细胞于s期,增加Caspase-3蛋白表达,抑制NF—KB通路的激活。董扬重庆医科大学附属第一医院黎友伦重庆医科大学附属第一医院中国肿瘤临床董扬;黎友伦.MLN4924促进人肺腺癌细胞株A549凋亡的机制研究.中国肿瘤临床.2012.2055-2058...
In AML, is regulated by NF-kB, the activity of which is in part controlled by neddylation. We demonstrate that MLN4924, a neddylation inhibitor presently used in clinical trials, decreases binding of NF-魏B to the promoter and downregulates in AML cells. This results in upregulation of the ...