cancer mostly occurred in the younger group;2. There was no difference in the expression of mismatch repair proteins MLH1,MSH2,MSH6 and PMS2 in the V gastric cancer tissues of proximal and distal gastric cancer;3. Patients with gastric cancer whose PMS2 expression was absent, the proporti...
·论著·联合检测 MLH1、MSH2、MSH6、PMS2 在结直肠息肉和结直肠癌中的表达差异及相关性研究张宁1 ,何洪芹 2(通讯作者 *)(1. 承德医学院,河北 承德;2. 沧州市人民医院,河北 沧州 )摘要: 目的 探讨错配修复基因 (Mismatch Repair,MMR)MLH1、MSH2、MSH6、PMS2 在结直肠息肉和结直肠癌中的表达差异及相关性...
Conclusion The rate of endometrial carcinoma in dMMR is low,and the loss of MLH1 and PM52 protein expression is more common than MSH2 and MSH6.The MLH1 and PMS2,MSH2 and MSH6 are often co-expressed or absent. MMR protein is closely related to the clinicopathological features of UCEC. ...
Taken together, enhanced tumoral MLH1 expression in MLH1-/PMS2-deficient colorectal carcinomas seems to be indicative of sporadic origin. In contrast, HNPCC-associated colorectal cancer showed absent or very weak MLH1 immunopositivity. Therefore, this semiquantitative and easy to exert MLH1 immunoscore...
Taken together, enhanced tumoral MLH1 expression in MLH1-/PMS2-deficient colorectal carcinomas seems to be indicative of sporadic origin. In contrast, HNPCC-associated colorectal cancer showed absent or very weak MLH1 immunopositivity. Therefore, this semiquantitative and easy to exert MLH1 immunoscore...
MSH6 or PMS2 screening for point mutations by pre-screening or direct sequencing, and for large genomic anomalies by MLPA (in case of negative results for MSH2/MLH1 screening, according to the tumour MMR status). MLH1 promoter methylation characterisation by MSP, bi- sulphite pyrosequencing (...
hMutLα is a heterodimer of the human MutL homologues hMLH1 and hPMS2, and we decided to exploit its asymmetry to study this function. We now show that although the two subunits contribute differently to the ATPase activity of the heterodimer, hMutLα variants in which one subunit was able...
In127 cases of colorectal polyps, hMLH 1 the deletion rate of hMSH2 protein, hMSH6 protein and hPMS2 protein were 9%(11/127),2%(3/127), 4.7%(6/127) and 15.7%(20/127) respectively. Conclusion The expression of mismatch repair protein was absent in some colorectal polyps and had no ...
Furthermore, hMSH2, hMLH1, and hPMS2 can be co-immunoprecipitated in the presence of DNA and ATP (11). Another MutL protein, theSaccharomyces cerevisiae MLH3 was shown to interact with MLH1 in a two-hybrid system (2). Similarly, the association of hMLH1 and hMLH3 was described in ...
Genetic testing of PMS2 gene was performed, but no mutation was identified. MLH1 promoter hypermethylation was detected in tumor DNA and in DNA extracted from PBLs, leading to the diagnosis of constitutional epimutation. Epigenetic testing was offered to other family members and the epimutation ...