Because T cells play a key role in the rejection of allogeneic stem cells, most transplantation studies have focused on the induction of T-cell tolerance. Natural killer (NK) cells represent another barrier that prevents engraftment following transplantation over MHC barriers. Because of their ...
With unequal starting conditions the larger population dominated when resting cells were used, but small populations of preactivated cells or separated CD8 + cells could also dominate. Depletion of CD16 + natural killer (NK) cells and of CD2 cells (B cell and monocytes) had no effect on ...
Decreased enrichment was demonstrated in gene signatures associated with immune or inflammatory pathways, including natural killer cell cytotoxicity, complement activation, and interferon-gamma signaling (Supplementary Fig. 5). Fig. 2: The MPAL transcriptional signature is stem-like, on the continuum of...
Summary To clarify the immune response of CD57 + T cells (most of them are CD8 + ) in peripheral blood (PB) against alloantigens in order to elucidate the T helper 1 (Th 1) immune response, we assessed the role of CD57 + T cells in IFN- γ (one of the representative Th 1 cyt...
Cell separations were performed using monoclonal antibodies and a fluorescence-activated cell sorter (Vantage FACSSORT; Becton Dickinson, San Jose, CA). Granulocytes were first separated from mononuclear cells by a Ficoll cut (specific gravity, 1.074) and then further purified using the monoclonal anti...
depleted spleen cells, donor CD8+T cells, and donor G-CSF-mobilized peripheral blood mononuclear cells. The conditioning regimen is clinically acceptable and can be used for treating hereditary hematological diseases and autoimmune diseases, as well as for promoting organ transplantation immune tolerance...
These results show that cloned T cells with PLT, CML, natural killer or suppressor activities, derived from MLC, can act as valuable tools in the elucidation of T cell function in vitro. To this end, the correlation of defined function with analysis of cell surface markers using monoclonal ...
For example, melanoma derived exosomes can mediate immune suppression [2]. They can directly interact with and suppress natural killer and cytotoxic CD8+ lymphocytes or induce myeloid derived suppressor cells (MDSCs). MDSCs can anergize anti-tumor CD8+ lymphocytes, promote M2 macrophage (Mφ) ...
Next, we used models of mixed type 1-type 2 inflammation to test the cell-intrinsic impact of IFNγ on activated ILC2s and IL-5+ Th2 cells. First, we used a reductionist approach, co-administering the cytokines IFNγ and IL-33. Exogenous IFNγ directly restricted IL-33-driven ILC2 exp...
After stimulation with DCleu ex vivo, leukemia-specific antileukemic immune cells are activated. Therefore, Kit-M treatment may be an attractive immunotherapeutic tool to treat patients with myeloid leukemia. Kit-M-mediated antileukemic effects on whole bone marrow (WBM) were evaluated and compared ...