-free image to be restored), g represents the observed images that must be dealt with during detection, C is a constant matrix representing the additive noise during imaging, ⊛ denotes the convolution operation, and P is the point spread function (PSF) which corresponds to the microscope:...
Images were analyzed using a Leica TCS SP5 confocal microscope (Leica Mycrosystems, Wetzlar, Germany). Western blotting. Protein samples were separated by 4–20% SDS-PAGE (Bio-Rad), transferred to nitrocellulose membranes (Whatman, Dassel, Germany) and blocked with 3% non-fat milk/PBS, 0.1%...
How long have you been taking photographs through a microscope? What first sparked your interest in photomicrography? More than 20 years. I had an undergraduate advisor who was passionate about imaging flatworms. He introduced me to electron and fluorescence microscopy. This was in the lat...
The immunostained cells were examined under a confocal laser scanning microscope (FV1000; Olympus, Tokyo, Japan) or a fluorescence microscope (BZ-9000; KEYENCE, Osaka, Japan). Chromosome spread Transfected cells were selected with puromycin for 48 h and treated with 100 nM nocodazole (Sigma...
labeled and control HeLa cells were then injected subcutaneously into the left and right rear flank, respectively, of three separatemice(injection volume: 5 × 106/100 μl). Time-dependent fluorescence images were acquired with a Leica M205 FA fluorescentstereo microscopeusing a 3.95× objective ...
By using fluorescently labeled phalloidin we have examined, at the light microscope level, the three-dimensional distribution and reorganization of actin-l... RW Seagull,MM Falconer,CA Weerdenburg - 《Journal of Cell Biology》 被引量: 312发表: 1987年 Evidence for kinesin-related proteins in the...
Confocal microscopy of fixed and immunolabeled coverslips was carried out using a Leica SP2 laser scanning confocal microscope. Captured images were adjusted in Adobe Photoshop CS2 version 9.0. Live cell imaging Live cell imaging was performed using a Nikon BioStation IM CELL-S1 microscopy system (Ni...
overlay of both channels (DAPI in blue and GFP in green) is displayed. Images are taken in 2048 × 2048 resolution using a widefield microscope Olympus FX81 with 60x oil objective with post-processing using HuygensPro20.10. Effects of KDM6A variants in SW-1710 cells is shown in Fig....
The cells were monitored on an inverted microscope (Axio Observer.Z1/7, Carl Zeiss) using an LD Plan-NEOFLUAR 20x NA 0.4 Korr Ph2 M27 objective lens. Images were captured every 2 min for 48 h using a digital camera (ORCA, Hamamatsu) and were processed by the ZEISS ZEN2 image ...
Images were taken on a DeltaVision microscope (Applied Precision) as previously described42. For z-stacks, images were taken at an interval of 0.4 μ m by Softworx software (Applied Precision). The single-plane images were deconvoluted using Softworx software (Applied Precision). Then the...