本试剂盒内含Mtphagy Dye (用于检测线粒体自噬) 和Lyso Dye (溶酶体染料)。Mtphagy Dye通过化学结合,固定在细胞内的线粒体上,会发出较弱的荧光。当线粒体发生自噬,损伤的线粒体会与溶酶体融合,pH会下降,变成酸性,此时Mtphagy Dye会产生较强的荧光。如想直观观察Mtphagy Dye标记的线粒体和溶酶体的结合,可...
●使用Hanks’ HEPES缓冲液洗涤两次 ●添加100 nmol/l Mtphagy Dye working solution孵化30分钟 ●使用Hanks’ HEPES 缓冲液洗涤两次 ●在含有10 μmol/l CCCP的培养基培养24小时 ●通过荧光显微镜观察到发生自噬后,去除含有CCCP培养基,添加1 umol/l Lyso Dye working solution孵化30分钟 ●使用Hanks’ HEPES 缓冲...
本试剂盒内含Mtphagy Dye (用于检测线粒体自噬) 和Lyso Dye (溶酶体染料)。Mtphagy Dye通过化学结合,固定在细胞内的线粒体上,会发出较弱的荧光。当线粒体发生自噬,损伤的线粒体会与溶酶体融合,pH会下降,变成酸性,此时Mtphagy Dye会产生较强的荧光。如想直观观察Mtphagy Dye标记的线粒体和溶酶体的结合,可联...
本试剂盒内含Mtphagy Dye (用于检测线粒体自噬) 和Lyso Dye (溶酶体染料)。Mtphagy Dye通过化学结合,固定在细胞内的线粒体上,会发出较弱的荧光。当线粒体发生自噬,损伤的线粒体会与溶酶体融合,pH会下降,变成酸性,此时Mtphagy Dye会产生较强的荧光。如想直观观察Mtphagy Dye标记的线粒体和溶酶体的结合,可...
Mitophagy dye is a pH-sensitive probe that localizes to mitochondria. Red puncta indicate engulfed mitochondria in an acidic lysosomal environment26. NPCs were incubated with 0.1 μmol/L mitophagy dye for 30 min at 37 °C prior to treatment to load the probes. Finally, the treated ...
Mitophagy Detection 無需經轉染作用表現蛋白,讓偵測mitophagy變簡單! 用於活細胞影像偵測 操作簡單一僅需使用Mtphagy Dye即可偵測mitophagy 附有Lyso Dye ,可用於Co-staining,偵測lysosome Cat. No.ProductSizeEx/Em (nm) MD01Mitophagy Detection Kit 線粒體自噬偵測套組1 setMtphagy Dye: 530 / 700 ●...
●通过荧光显微镜观察到发生自噬后,去除含有CCCP培养基,添加1 umol/l Lyso Dye working solution孵化30分钟 ●使用Hanks’ HEPES 缓冲液洗涤一次后,通过荧光显微镜观察被Mtphagy Dye和Lyso Dye联合染色。 Mtphagy Dye・Lyso Dye的激发光谱和荧光光谱 Mtphagy Dye...
Colocalization between the mitophagy dye and the lysosome dye were quantified (F) with a representative set images shown in panel G. (H,I) Assessment of mitophagy in vehicle- and tomatidine-treated human neural cells. Colocalization between the mitophagy dye and the lysosome dye were quantified...
Most commonly, LC3 fused with a fluorescent protein (e.g., GFP-LC3) serves as the marker of the autophagosome and can be coupled with use of a fluorescent dye (e.g., Mitotracker Red) to specifically label mitochondria. Co-localization of the two fluorescent signals would be interpreted as...
in combination with the lysosomal dye LysoTracker Red (Elmore et al., 2001). Following on from this, the co-localisation of fluorescently labelled mitochondria with the autophagosomal marker LC3B, or endo/lysosome markers such asCD63, have been used as a readout for mitophagy (Bampton et al....