同仁化学 线粒体自噬—Mitophagy Detection Kit 货号:MD01 线粒体自噬检测试剂盒 规格: 1set ¥4060.00 商品信息: 储存条件:0-5度保存,避光 运输条件:室温 产品概述: 线粒体 (Mitochondria) 是细胞中重要的细胞器之一,可以为细胞活力提供能量 。近年有报道去极化线粒体的积累引起的阿尔茨海默病 (Alzheimer's...
用羰基氰化物间氯苯腙(CCCP,一种线粒体解偶联剂)诱导Parkin表达的HeLa细胞线粒体自噬,并使用线粒体自噬检测试剂盒(Mitophagy Detection Kit:MD01)和线粒体膜电位检测试剂盒(JC-1 MitoMP Detection Kit:MT09)观察荧光结果。 结果证实在未经CCCP处理的细胞中几乎未检测到线粒体自噬的发生,并且线粒体膜电位正常维持。
用羰基氰化物间氯苯腙(CCCP,一种线粒体解偶联剂)诱导Parkin表达的HeLa细胞线粒体自噬,并使用线粒体自噬检测试剂盒(Mitophagy Detection Kit:MD01)和线粒体膜电位检测试剂盒(JC-1 MitoMP Detection Kit:MT09)观察荧光结果。 结果证实在未经CCCP处理的细胞中几乎未检测到线粒体自噬的发生,并且线粒体膜电位正常维持。
Mitophagy Detection 無需經轉染作用表現蛋白,讓偵測mitophagy變簡單! 用於活細胞影像偵測 操作簡單一僅需使用Mtphagy Dye即可偵測mitophagy 附有Lyso Dye ,可用於Co-staining,偵測lysosome Cat. No.ProductSizeEx/Em (nm) MD01 Mitophagy Detection Kit 線粒體自噬偵測套組 1 set Mtphagy Dye: 530 / 700 ● ...
無需經轉染作用表現蛋白,讓偵測mitophagy變簡單! 用於活細胞影像偵測 操作簡單一僅需使用Mtphagy Dye即可偵測mitophagy 附有Lyso Dye ,可用於Co-staining,偵測lysosome Cat. No.ProductSizeEx/Em (nm) MD01Mitophagy Detection Kit 線粒體自噬偵測套組1 setMtphagy Dye: 530 / 700 ● ...
The mitophagy in HEI-OC1 cells was assessed using the mitophagy detection kit(DOJINDO, MD01). On a nutshell, HEI-OC1 cells were seeded onto glass slides in a six-well plate. Subsequently, the working solution of 100 nM Mtphagy Dye was added to the wells and incubated with the cells...
www.nature.com/scientificreports OPEN Loss of O6-methylguanine DNA methyltransferase (MGMT) in macrophages alters responses to TLR3 stimulation and enhances DNA double-strand breaks and mitophagy Md Fazlul Haque1,2, Salisa Benjaskulluecha1,3, Atsadang Boonmee1,4, Pornrat Kongkavitoon1,3...
For determining GSH levels, the GSH Assay Kit (A006-2–1, Nanjing Jiancheng Bioengineering Institute, China) was applied. Cell lysates, centrifuged at 12,000 g, 4 °C for 10 min, served as the starting material. The GSH detection working solution was added and thoroughly mixed. Following ...
The enzyme pair PINK1 and PRKN together orchestrates a cytoprotective mitophagy pathway that selectively tags damaged mitochondria with phospho-serine 65 ubiquitin (pS65-Ub) and directs them for autophagic-lysosomal degradation (mitophagy). We previously
TUNEL assay Apoptotic cells were detected by a TUNEL detection kit (KeyGen Biotechnology Co., Ltd, Jiangsu, China) accord- ing to the manufacturer's protocol [17]. Briefly, cells were fixed with 4% paraformaldehyde for 30 min and permea- bilized with 1% Triton X-100 for 5 min at ...