用羰基氰化物间氯苯腙(CCCP,一种线粒体解偶联剂)诱导Parkin表达的HeLa细胞线粒体自噬,并使用线粒体自噬检测试剂盒(Mitophagy Detection Kit:MD01)和线粒体膜电位检测试剂盒(JC-1 MitoMP Detection Kit:MT09)观察荧光结果。 结果证实在未经CCCP处理的细胞中几乎未检测到线粒体自噬的发生,并且线粒体膜电位正常维持。
同仁化学 MD01 线粒体自噬—Mitophagy Detection Kit参考价 ¥ 4060 订货量 ≥1SET 具体成交价以合同协议为准 公司名称 北京沃比森科技有限公司 品牌 DOjinDO 型号 产地 厂商性质 生产厂家 更新时间 2024/12/17 18:29:45 访问次数 1945 产品标签 同仁化学MD01线粒体自噬检测试剂盒 在线交流 发送...
3.自噬诱导和线粒体膜电位变化关系的检测 用羰基氰化物间氯苯腙(CCCP,一种线粒体解偶联剂)诱导Parkin表达的HeLa细胞线粒体自噬,并使用线粒体自噬检测试剂盒(Mitophagy Detection Kit:MD01)和线粒体膜电位检测试剂盒(JC-1 MitoMP Detection Kit:MT09)观察荧光结果。 结果证实在未经CCCP处理的细胞中几乎未检测到...
Dojindo的DALGreen為一個可穿透細胞膜的綠色螢光染劑(λex : 405 nm/ λem : 525 nm)。它具有可在疏水(hydrophobic)及酸性條件下發出螢光的特性,因此可被用來來偵測自體溶酶體(Autolysosomes)。可用於螢光顯微鏡的活細胞影像觀測或用於流式細胞儀進行定量分析。 原理 在自噬作用的過程中,當自噬小體(Autophagos...
To detect mitophagy, a Mitophagy Detection Kit (Dojindo Molecular Technologies) was used as described previously33. In brief, cells were washed twice with DMEM and incubated with 100 nM Mtphagy Dye diluted in DMEM for 30 min at 37 °C. After incubation, cells were washed twice with ...
Mitochondrial membrane potential was assessed using a JC-1 MitoMP Detection Kit (Dojindo Molecular Technologies, Japan) in accordance with the manufacturer’s instructions. Briefly, treated NPCs were incubated in preformulated JC-1 working solution at 37 °C in a 5% CO2 incubator for 30 min...
Mitophagy Detection Kit 发表于2024年4月14日由Wako和光纯药中国 Mitophagy Detection Kit 品牌:Dojindo CAS No.: 储存条件:2-10℃ 纯度:– 产品编号 (生产商编号) 等级规格运输包装零售价(RMB)库存情况参考值 344-91901 –1 set–咨询–– * 干冰运输、大包装及大批量的产品需酌情添加运输费用 ...
2.8. Mitophagy detection assay The mitophagy in HEI-OC1 cells was assessed using the mitophagy detection kit(DOJINDO, MD01). On a nutshell, HEI-OC1 cells were seeded onto glass slides in a six-well plate. Subsequently, the working solution of 100 nM Mtphagy Dye was added to the wells...
DAPI was incubated with 2% bovine serum albumin at room temperature for 2 h, and the cells were stained using a Dojindo Mitophagy Detection Kit (Dojindo EU GmbH, Munich, Germany). After staining, the cells were visualized with a Zeiss LSM 510 META confocal microscope (Heidelberg, Germany)...
Induction of apoptosis was determined with the Annexin V, FITC Apoptosis Detection Kit (DOJINDO, #AD10) according to manufacturer's instructions. Loss of mitochondrial membrane potential was evaluated with TMRM (100 mM, Thermo Fisher, #T668). Mitochondrial ROS were detected with MitoSOX (5 µ...