粪便miRNA-92a表达与粪便隐血试验联合诊断结直肠癌的应用
机制上,研究人员发现PCa外泌体递送的microRNAs (miRNAs)在骨稳态中起着关键作用。在递送的miRNAs中,最丰富的miRNA miR-92a-1-5p通过直接靶向COL1A1来下调I型胶原蛋白的表达,从而促进破骨细胞分化并抑制成骨细胞生成。此外,PCa外泌体也显著降低了体内I型胶原蛋白的表达。 图2. PCa外泌体调节骨稳态、骨ECM重塑...
Through luciferase assays, we found evidence that IBSP is a miRNA-92a-3p target gene that negatively regulates osteoblast differentiation. Moreover, the present study confirmed that pre-injection of agomiR-92a-3p leads to increased bone formation. Collectively, these results indicate that miRNA-92a-...
miRNA-92a在缺血再灌注肾损伤中的表达变化
在筛查现场样本中验证粪便样本miRNA-92a表达量与结直肠肿瘤诊断的关系,并初步分析了miRNA-92a与大便隐血联合检测用于结直肠癌筛查的诊断价值.本研究共收集到41例临床结直肠癌患者,42例筛查现场腺瘤患者和43例筛查现场正常对照.以离心过滤法纯化粪便样本中所有1miRNA.U6小RNA为内参基因,用荧光定量PCR法检测miR-92a表达...
分类号:R72密级:公开MiRNA-92a可下调TrkA蛋白表达并促进神经母细胞瘤增殖和迁移MiRNA-92amaydownregulateTrkAexpressionandpromotetheproliferationandmigrationofhumanneuroblastomacells作者姓名:***科专业:儿科学导师:**勤答辩委员会主席:论文答辩日期:二〇一三年五月二十一日院校地址:北京市复兴路28号邮政编码:100853解放军...
［摘要］目的探讨血清微小RNA(miRNA)-196a、miRNA-92a表达水平与宫颈癌根治术后复发的关系。方法选取2015年1月至2016年6月甘肃省肿瘤医院收治的113例行宫颈癌根治术患者为宫颈癌组,68名体检健康女性为对照组,qRT-PCR检测两组血清miRNA-196a、miRNA-92a表达。分析宫颈癌患者血清miRNA-196a、miRNA-92a水平与临床病理...
MicroRNA-92a negatively regulates TLR-triggered inflammatory response in macrophages by targeting MKK4 In this study, we have found that the stimulation of multiple TLRs rapidly reduced the levels of microRNA-92a and some other members of the miRNA-92a family in macrophages. MiR-92a mimics signifi...
【结论】成功构建糖尿病大鼠ED模型,miRNA-92a抑制剂antagomir可以显著改善糖尿病ED大鼠的勃起功能,降低miRNA-92a的表达水平.【目的】在组织水平研究miRNA-92a抑制剂改善糖尿病ED大鼠勃起功能的机制.【方法】取冻存的大鼠阴茎海绵体组织,提取组织的总蛋白或RNA,或者取大鼠阴茎海绵体组织的石蜡包埋切片或冰冻切片,进行本...