#plot_MA_and_Volcano(log2(res$baseMean+1), res$log2FoldChange, res$padj)#dev.off()
Results MPS detected 432 miRNAs; however, only 90 miRNAs passed through the selection criteria (p<0.05, BaseMean>10, the difference in log2FC>0.5). We selected 45 miRNAs for validation based on the selection criteria and the literature. We validated miR-1-3p (p=0.006, FC=1.757) to be ...
Also, to filter out the candidates with extremely low expression levels, we further selected the mean of normalized counts (baseMean) >1 for miRNAs and >10 for genes. Survival analysis and the additive effect on survival of miRNAs To define clinically relevant candidates, another R package, ...
(A) Expression patterns of selected abnormal isomiRs from the two gene families across 16 cancer types (isomiRs with an RPM value not less than 50 and a baseMean value not less than 500 in at least one cancer type were selected). The log2FC distribution was estimated based on the ...