Patients with medication-refractory epilepsy frequently benefit from vagus nerve stimulation, a treatment with an expanding array of other medical uses. Vagus nerve stimulation treatment can result in side effects including a cough, vocal adjustments, the tightening of vocal cords, the uncommon ...
This array design is a powerful tool for studying the role of small non-coding RNAs and their involvement in a broad spectrum of developmental and physiological mechanisms.To keep pace with the discovery of new and novel miRNA, we are pleased to offer the GeneChip™ miRNA 4.0 Array to our...
参考文献: [1]Yingqing S, Seongjoon K, Neill W, et al.Development of a micro-arrayto detect human and mouse microRNAs and characterization of expressionin human organs. Nucleic Acids Res,2004,32 (22): 2486～2494 [2]Caifu C, Kelly M, Ada H.W, et al. Real-time PCR:Advancing RNAInte...
Mouse miRNA Array(1) Add 100 µl Bead washing buffer directly to 20 µl ligation reaction mix from Step 3, place the tube on the magnetic stand for 30 seconds, and aspirate the buffer.(2) Remove the tube from the magnetic stand and add 20 µl of Extension mix to resuspend the ...
PCR Array: 炎症相关PCR Array 文章整体解读 研究背景 复发性流产(RPL)是一个主要的妊娠问题,其确切病因和发病机制尚不完全清楚。 最近的进展突出了利用 miRNA 分析和表达作为 RPL 可靠生物标志物的实用性,本研究探讨了RPL 病例和经产对照女性血浆中 miRNA 的表达谱,以探索 miRNA 作为 RPL 可靠预测生物标志物的潜...
1pM Figure4.a)ConcentrationdependentfluorescencesignalsforfourmiRNAs(humanLet-7a,miR21,miR96,andmiR206).FluorescenceimagesofhumanLet7a(10fm-10pm)arraydetectionareshown.b)Fluorescencemicro-arraydetectionoffivehumanmiRNAsfromhumanskeletalmuscle(left)orheart(middle)totalRNAextracts(2mg).SyntheticmiR96(1pM)was...
此外,级联Toehold介导的DNA链置换信号扩增可以灵敏地检测低丰度的miRNA(低至241.56pM)。该研究以题为“Microneedle Array Encapsulated with Programmed DNA Hydrogels for Rapidly Sampling and Sensitively Sensing of Specific MicroRNA in Dermal Interstitial Fluid” 的论文发表在 ACS NANO 期刊上。
这里选择的文章是2014年发表的,作者用ET-1刺激human iPSCs (hiPSC-CMs) 细胞前后,观察miRNA和mRNA表达量的变化,我并没有细看文章的生物学意义,仅仅从数据分析的角度解读一下这篇文章,mRNA表达量用的是Affymetrix Human Genome U133 Plus 2.0 Array,分析起来特别容易。得到表达矩阵,然后用limma这个包找差异表达基因...
Order the pre-designed array with 238,000 SNPs and indels today Customize by adding up to 170,000 de novo markers of your choosing or select from the Axiom database of genotype-tested markers Watch a case-control study presentation Case-control study of microRNA target site...