miRNA expression arrayPNAPNA arrayMicroRNAs (miRNAs) are short, non-coding RNAs that play a critical role in development, metabolism and other fundamental biological processes, and are also important in diseases such as cancer. To study miRNA expression levels in a systematic and parallel manner, ...
Given that the expression of specific miRNAs has been linked with several diseases [1−5], the research group wanted to address the potential involvement of miRNA in pancreatic cancer. The first step in this study was to isolate good quality RNA from normal and diseased ...
The 20X Hybridization Controls are spiked into the hybridization cocktail, independent of RNA sample preparation, and are thus used to evaluate sample hybridization efficiency on eukaryotic gene expression arrays. As the 20X Hybridization C...
[1]Yingqing S, Seongjoon K, Neill W, et al.Development of a micro-array to detect human and mouse microRNAs and characterization of expression in human organs. Nucleic Acids Res,2004,32 (22): 2486～2494 [2]Caifu C, Kelly M, Ada H.W, et al. Real-time PCR:Advancing RNA Interference...
方法:定量PCR技术是检测基因的表达水平的金标准,很多科研文章研究发现miRNA也能影响靶基因的mRNA水平含量,利用靶基因的PCR Array就能够同时检测很多靶基因的表达水平。 以下这篇文章通过LncRNA PCR阵列筛选miR-21的靶基因,找到GAS5是miR-21的靶点。 Zhang Z, Zhu Z, Watabe K, et al. Negative regulation of lnc...
Cardiovascular Disease miScript miRNA PCR ArrayDogmiRNA ExpressionMIFD-113Z 心血管疾病miRNA PCR芯片可同时检测84个与心血管疾病相关的miRNA表达,miRNA列表如下: Myocardial Infarction: Up-Regulated: let-7e-5p, miR-122-5p, miR-126-5p, miR-133b, miR-145-5p, miR-146a-5p, miR-15b-5p, miR-208a,...
along with heightened accumulation of proline, soluble sugars, and flavonoids. Along these lines, enhanced GSH activity and GST gene expression levels promoted the detoxification of cadmium. The consequence of these defensive mechanisms was a decrease in the levels of Cd2+, MDA, and H2O2 present ...
Fractions enriched in small RNAs can be used in downstream miRNA expression analyses, and are particularly useful for Northern blotting and solution hybridization assays (RPA). For miRNA array analysis applications, we recommend isolating total RNA (including the small RNA ...
across the top 25 most differentially expressed miRNAs. The miRNA clustering tree is shown on the left and the samples’ clustering tree on the top of the graph. There was no significant difference in the miRNA expression pattern between patients with Type 1 diabetes and those with Type 2 ...
MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant WomenMiRNA expression analysis in plasma and placenta biopsies by PCR array. Analyses of miRNA with antiviral (A,C) and with immunological function (B,D) was performed on plasma (A,B)