使用币种: 人民币 采购类型: 线上单 经费负责人审批时间: 2024-11-22 供应商商品名称货号规格单价数量总价 武汉枢密脑科学技术有限公司 rAAV-CAMKIIa-mCherry-5'miR-30a-shRNA(CCR5)-3’-miR30a-WPREs质粒 RT-140-241104 200ul 8264.8 1.0 8264.8热门...
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For transfection, arachnoid cells were seeded into six-well plates and then infected with the constructed AP1 shRNA lentiviruses and control shRNA lentiviruses at an MOI of 30. After 8 h, the supernatant was moved, and fresh medium was added. Then, the cells were put back into the ...
Lentiviruses expressing CLCF1 shRNA or miR-30a-5p at high titer were procured from GeneChem (Shanghai, China). Lentiviruses were incubated with target cells in media supplemented with polybrene (8 μg/mL) for 24 hours. After transduction, puromycin (1 μg/mL) was added for the ...