目的:探讨在体过表达miR-30a对实验性自身免疫性脑脊髓炎(EAE)模型小鼠脱髓鞘病变的作用.方法:构建含稳定表达miR-30a的shRNA慢病毒载体,以含有空载体的慢病毒为对照(LV-ctrl).雌性C57BL/6小鼠随机分为正常对照组(normal),对照慢病毒(LV-ctrl)组和miR-30a慢病毒(LV-miR-30a)组,2组慢病毒经尾静脉注射,注射7d...
使用币种: 人民币 采购类型: 线上单 经费负责人审批时间: 2024-11-22 供应商商品名称货号规格单价数量总价 武汉枢密脑科学技术有限公司 rAAV-CAMKIIa-mCherry-5'miR-30a-shRNA(CCR5)-3’-miR30a-WPREs质粒 RT-140-241104 200ul 8264.8 1.0 8264.8热门...
*** rAAV-CAMKIIa-mCherry-5'miR-30a-shRNA(CCR5)-3’-miR30a-WPREs质粒 RT-140-241104 200ul 8264.80 1 8264.80 招标进度跟踪 2024-11-22 招标公告中山医学院rAAV-CAMKIIa-mCherry-5'miR-30a-shRNA(CCR5)-3’-miR30a-WPREs质粒 当前信息 招标项目商机 暂无推荐数据 400-688-2000 欢迎来电咨询...
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For transfection, arachnoid cells were seeded into six-well plates and then infected with the constructed AP1 shRNA lentiviruses and control shRNA lentiviruses at an MOI of 30. After 8 h, the supernatant was moved, and fresh medium was added. Then, the cells were put back into the ...
Lentiviruses expressing CLCF1 shRNA or miR-30a-5p at high titer were procured from GeneChem (Shanghai, China). Lentiviruses were incubated with target cells in media supplemented with polybrene (8 μg/mL) for 24 hours. After transduction, puromycin (1 μg/mL) was added for the ...
To investigate the effect of FOXD1 expression on ovarian cancer cells, five ovarian cancer cell lines, including A2780, HO8910, SKOV3, OVCAR3, CAOV3 and one lung cancer cell line, H1299, were employed and transfected with a FOXD1 overexpression vector or an shRNA targeting FOXD1. As ...
Concurrently, shHIF-1α-knockdown treatment in MDA-MB-231–pLKO/miR-622 cells administered the miR-622 inhibitor led to a significant reduction of more than 50% in migration and invasion activity compared to negative control (shRNA, ctrl) in the transwell assay (Figure 4B,C). This ...
In a subset of experiments, ASMCs were transduced with Ad.TRAF3IP2 shRNA or Ad.GFP shRNA (moi10 for 48 h) prior to IL-18 addition at 10 ng/mL for 48 h. To determine if the ectopic expression of RECK inhibits IL-18-induced ASMC proliferation, ASMCs were transduced with Ad.RECK ...