Among them, hyper-responders show significantly lower T-cell ubiquitin ligand-2 (TULA-2) mRNA level and higher miR-148a-3p and miR-25-3p levels than hyporesponders. TULA-2, also known as UBASH3B, is a known pro
结果发现,Anti-miR-148a抑制了细胞增殖、细胞周期的前进,细胞迁移、在软琼脂的停泊非依赖性生长及SCID老鼠皮下肿瘤的形成。 引入anti-miR-148a提升了PTEN蛋白及mRNA的表达,这表明了miR-148a对PTEN的定向作用。 然而,当与PTEN mRNA 3′UTR报告基因突变体联合转染时,Anti-miR-148a却不能抑制PTEN的表达 。 实验还...
图2:miR-148a是体外破骨细胞生成的正调控因子。 A,在RANKL刺激的破骨细胞分化过程中,用RT-qPCR检测miR-148a和NFATc1的表达量。 B,采用RT-qPCR检测miR-148a mimics/inhibitors的转染效率。 C,BMMs转染miR-148a类似物/抑制物及...
Here we found miR-148a-deficient mice to be more susceptible to colitis and colitis-associated tumorigenesis. Both were associated with increased nuclear factor κB (NF-κB) and signal transducer and activator of transcription 3 (STAT3) signaling. Bone marrow- and non-bone marrow-derived miR-148...
Glioma cells miR-148a Proliferation Metastasis CADM1 Mots clés Cellules de gliome miR-148a Prolifération Métastase CADM1 Introduction Glioblastoma (GBM) is a very lethal malignant brain tumor (grade IV astrocytoma according to WHO classification) [1]. Although surgical resection, chemotherapy and ...
pMSCV-miR-148a遥并测得病毒滴度为5伊10 8 CFU/ml遥结论以miR-148a前体序列构建的逆转录病毒表达载体 pMSCV-miR-148a能够产生高滴度的逆转录病毒袁为深入研究miR-148a的生物学功能奠定了基础遥 关键词院has-miR-148a曰DNA甲基转移酶曰肿瘤曰甲基化曰逆转录病毒 ...
织中的miR-34a、miR-148a及Wntl的mRNA含量。 3、荧光定量PCR(qRT-PCR):以人正常乳腺组织和乳腺癌组织总RNA为模板, 反转录后得到miR-34a、miR-148a、WntlmRNA的eDNA模板;利用SYBRGreen Mix和特异性PCR引物对miR-34a、miR-148a、WntlmRNA相对表达量进行检测 ...
significantly increased in the tumor compared to matched normal healthy tissue, while miR-148a levels are decreased. Functional analysis demonstrated post-transcriptional regulation of TFR1 by miR-148a in HCC cells as well as decreased HCC cell proliferation upon either miR-148a overexpression or ...
文中作者发现敲低ATP7A可以增强顺铂对乳腺癌细胞的毒性。在浸润性乳腺癌细胞中过表达miR-148a-3p可以增加癌细胞对顺铂的敏感性,继而促进DNA的损伤和细胞凋亡。不仅如此,作者还发现ATP7A是miR-148a-3p新的靶基因。总之,文章证明miR-148a可以通过抑制ATP7A表达从而促进化疗导致的乳腺癌细胞凋亡。
Conversely, miR-148a overexpression promoted cell proliferation and cell cycle progression. p27, a key inhibitor of cell cycle, was verified as the target of miR-148a, indicating miR-148a might downregulate p27 expression to promote gastric cell proliferation. Moreover, we confirmed that miR-...