结论Hsa-miR-148a-3p 通过抑制DUSP1的表达而作为癌基因发挥作用，这有助于开发针对乳腺癌的新型治疗靶点。关键词：Hsa-miR-148a-3p ；DUSP1；乳腺癌；促癌Abstract:Objective To investigate the role of Hsa-miR-148a-3p in regulating biological behaviors of breast cancer cells and explore the mechanism....
Hsa-miR-148a-3p 能与 DUSP1 结合;RT-qPCR 结果表明,Hsa-miR-148a-3p 在乳腺癌组织和细胞中显著上调(P<0.01),而 DUSP1 则显著下调(P<0.01).细胞实验结果表明,Hsa-miR-148a-3p 促进乳腺癌细胞增殖,迁移和侵袭,抑制细胞的凋亡(P<0.01).动 物实验结果表明,Hsa-miR-148a-3p 促进小鼠体内肿瘤质量和体积...
摘要 目的通过生信分析结合体内外实验验证Hsa-miR-148a-3p调控双特异性磷酸酶1(DUSP1)对乳腺癌细胞生物学行为的影响。方法TCGA数据库下载数据并鉴定差异微小RNA(miRNA)和信使RNA(mRNA);数据库...展开更多 Objective To investigate the role of Hsa-miR-148a-3p in regulating biological behaviors of breast ...
miR-125bInfant formulaLactationMilk-derived microRNAs (miRNAs), including hsa-miR-148a-3p (miR-148a) and hsa-miR-125b-5p (miR-125b), have been shown to be beneficial to the gastrointestinal function in infants. Here, we investigated their expression during lactation in humans and determined...
细胞实验结果表明,Hsa-miR-148a-3p促进乳腺癌细胞增殖,迁移和侵袭,抑制细胞的凋亡(P<0.01).动物实验结果表明,Hsa-miR-148a-3p促进小鼠体内肿瘤质量和体积的增长(P<0.01),缩短小鼠的存活时间(P<0.01),免疫组化结果显示,Hsa-miR-148a-3p抑制DUSP1的表达水平(P<0.01).结论Hsa-miR-148a-3p通过抑制DUSP1的...
hsa-miR-148a-3pADH4Positive regulationAlcoholic hepatitisThe alcohol dehydrogenases (ADHs) and aldehyde dehydrogenases (ALDHs) play critical roles in alcoholism development and alcohol toxicology; however, few studies have focused on the miRNA-mediated mechanisms underlying the expressions of alcohol-...
目的:建立稳定的hsa-miR-148a-3p低表达人支气管上皮细胞株(16HBE).方法:根据miRBase中提供的序列信息设计hsa-miR-148a-3p tough decoy RNA(TuD RNA),并将其连接到慢病毒载体pLKO.1-puro上.将重组慢病毒载体转染至293FT细胞并包装为慢病毒后,收集病毒上清,感染正常16HBE细胞.用嘌呤霉素筛选出has-miR-148a-...
Moreover, we found hsa-miR-148a-3p significantly over-expressed in Intermediate and Late-Onset Friedreich Ataxia patients' group (IOG and LOG, respectively) compared to healthy individuals, indicating it as a putative prognostic biomarker in this pathology....