PAK2食管癌目的 探讨miR-545-3p抑制食管癌细胞Eca109和TE-1生长的机制.方法 以食管癌细胞Eca109和TE-1作为研究对象,转染miR-NC(对照组)或miR-545-3p(实验组);荧光实时定量聚合酶链反应(qRT-PCR)和蛋白质印迹法(Western blotting)检测细胞周期蛋白依赖激酶4(CDK4),细胞周期素D1 (Cyclin D1)和p21活化蛋白...
Taking together, we investigated the role of miR-545-3p in the process of HCC through regulating MT1M. 展开 关键词: miR-545-3p MT1M Hepatocellular carcinoma Invasion and migration DOI: 10.1016/j.biopha.2018.09.009 年份: 2018 收藏 引用 批量引用 报错 分享 ...
结果:过表达miR-140-3p后,A549和NCI-H1299细胞的增殖率升高,迁移能力下降;相反,抑制miR-140-3p的表达后,细胞的增殖率较低,但迁移能力提高。生物信息学分析结合实时定量反转录聚合酶链式反应验证提示,miR-140-3p可能靶向CBL基因,影响细胞生物学过程。结论:miR-140-3p对NSCLC细胞具有促进细胞增殖和抑制细胞迁移的...
Conclusion: Circ_0072088 promotes the proliferation and metastasis of NSCLC cells by regulating the miR-545-3p/STAT3 axis.项保利王布林卫佳于亚楠Chinese Journal of Cancer Biotherapy
Wnt信号通路目的:1.观察老年性骨质疏松症(SOP)人群与非SOP人群骨髓间充质干细胞(h BMSCs)来源外泌体(Exo)中mi R-545-3p表达的差异性;2.探究复方补肾活血颗粒(CKG)含药血清对SOP人群h BMSCs来源Exomi R-545-3p表达的影响;3.初步分析CKG通过h BMSCs-Exo途径调控Wnt通路对SOP人群h BMSC骨脂分化的影响.方法...
Gain-of-function assay elucidated that ectopic expression of miR-545-3p led to abolishment on the levels of osteogenic differentiation markers including OC, ALP and Runx2, as well as increase on the expression of SOST, a negative regulator of osteogenic differentiation. Meanwhile, we explained ...
(MTS法)和集落形成实验检测细胞增殖能力.结果过表达miR-545-3p后,CDK4,Cyclin D1和PAK2 mRNA及蛋白的表达明显下调(P<0.05),促进细胞周期的进展和细胞凋亡的增加(P<0.05),明显抑制食管癌细胞的增殖能力(P<0.05).结论miR-545-3p通过靶向干扰CDK4,Cyclin D1和PAK2的表达来抑制食管癌细胞的生长,是食管癌基因...
Circ_0018909 knockdown retarded the development of pancreatic cancer by modulating miR‐545‐3p to regulate FASN expression.doi:10.1002/jbt.23293Song, YinxueWang, JunXu, JingGao, YeXu, ZhichaoJournal of Biochemical & Molecular Toxicology
Plasma miR-34a-5p and miR-545-3p as Early Biomarkers of Alzheimer's Disease: Potential and Limitations. Mol Neurobiol. 2017 Sep;54(7):5550-62.Cosin-Tomas M, Antonell A, Llad? A, et al. Plasma miR-34a-5p and miR-545-3p as early biomarkers of Alzheimer's disease: potential and ...
In this study, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting were performed to analyze the expression of AFAP1-AS1, microRNA miR-545-3p, or G protein subunit beta 1 (GNB1). Cell Counting Kit-8 (CCK-8) and Transwell migration assays were used ...