MCE 国际站:hsa-miR-499a-5p antagomir 产品活性:hsa-miR-499a-5p antagomir 是一种经过特殊化学修饰的成熟miRNA的互补单链,全链进行甲氧基修饰,在5'端和3'端分别有2个和4个硫代骨架修饰,并在3'端连接有高亲和性胆固醇修饰。miRNA antagomir通过特异性结合成熟miRNA,阻止miRNA与其靶基因的互补配对,从而抑制mi...
miR-499a-5p靶向调控CDKN1A基因在心肌细胞 肥大中的作用机制研究 牟寒霜,黄震,郭婧 摘要目的:探讨miR-499a-5p靶向细胞周期蛋白依赖性激酶抑制剂1A(CDKN1A)抑制血管紧张素Ⅱ(AngⅡ)诱导的心肌细胞肥大的作用机制㊂方法:体外培养大鼠心肌细胞H9c2,并将H9c2细胞随机分为正常对照(Con)组㊁细胞肥大模型(AngⅡ)组㊁...
Taken together, these results indicate that miR-499a-5p may attenuate TNF-α-induced NPC apoptosis and an imbalance between anabolism and catabolism of the ECM by downregulating SOX4. 展开 关键词: Intervertebral disc degeneration Nucleus pulposus cell SOX4 miR-499a-5p ...
Material and methods The expression levels of circHIPK3, miR-499a-5p and E2F transcription factor 3 (E2F3) were measured by quantitative real-time PCR. Cell counting kit 8 assay and flow cytometry were performed to detect cell viability and apoptosis, respectively. Western blot analysis was emp...
Mechanistically, Circ_0001187 sponges miR-499a-5p to enhance the expression of E3 ubiquitin ligase RNF113A, which mediates METTL3 ubiquitin/proteasome-dependent degradation via K48-linked polyubiquitin chains. Moreover, we found that the low expression of Circ _0001187 is regulated by promoter DNA...
miR-135b-5p和miR-499a-3p的特异性引物,通过Real-Time PCR检测两种miRNA的表达.3,荧光素酶报告载体的构建:利用PCR技术扩增包含结合位点的MEF2C3'UTR区域,并将该区域连入荧光素酶报告载体,利用双荧光素酶报告试剂盒检测荧光强度的变化.4,Western blot:在HEK293和HUVEC细胞中分别转染miR-135b-5p和miR-499a-3p...
同时探讨miR-135b-5p和miR-499a-3p通过MEF2C对内皮细胞和血管平滑肌细胞的增殖和迁移等细胞行为的作用。研究方法1、血清中miRNA的获取:收集5m1动脉粥样硬化患者外周血,加入促凝剂,分离血清。利用Trizol分离方法,获取血清中miRNA.2、测定动脉粥样硬化患者血清中miRNA的水平:利用引物设计软件设计miR-135b-5p和miR-499...