Conclusion Overexpression of miR-499a-5p attenuates lung injury in rats with ARDS by targeting negative regulation of MMP-16 via activating the Nrf2 signaling pathway.Citation: DUAN Lingxiao, LI Qin, WU Changxue. miR-499a-5p attenuates lung injury in rats with acute respiratory distress syndrome...
Results miR-499a-3p and miR-499a-5p resulted to be overexpressed in CTNNB1 mutant EC patients at intermediate risk. Similarly, in the TCGA cohort, miR-499a-3p and miR-499a-5p were differentially expressed between CTNNB1 mutant and wild-type patients (p < 0.0001). NSMP patients with ...
miR-135b-5p和miR-499a-3p的特异性引物,通过Real-Time PCR检测两种miRNA的表达.3,荧光素酶报告载体的构建:利用PCR技术扩增包含结合位点的MEF2C3'UTR区域,并将该区域连入荧光素酶报告载体,利用双荧光素酶报告试剂盒检测荧光强度的变化.4,Western blot:在HEK293和HUVEC细胞中分别转染miR-135b-5p和miR-499a-3p...
Interestingly, miR-135b-5p and miR-499a-3p directly regulated a common target gene: myocyte enhancer factor 2C (MEF2C) which plays an important role in modulating cell phenotype of cardiovascular systems. Furthermore, our results indicated that the 2 elevated miRNAs could jointly promote ECs ...