目的 确定miR-373-3p是否参与去甲肾上腺素对结肠癌细胞RKO细胞增殖的调控. 方法 将结肠癌细胞RKO分为处理组和对照组,处理组细胞培养于含有终浓度10μmol/L去甲肾上腺素的培养基中,对照组培养于正常培养基中,使用MTT法检测细胞增殖的差异.使用划痕实验观察细胞迁移,使用流式细胞术检测细胞周期.通过RT-PCR检测处理组...
hsa-miR-373-3p inhibitor 英文名称: 总访问: 6 国产/进口: 进口 半年访问: 3 产地/品牌: MedChemExpress(MCE) 产品类别: 生化试剂 规格: 5 nmol; 20 nmol 最后更新: 2024-11-4 货号: HY-RI00826 CAS 号: 参考报价: 1200; 3360 立即询价 电话咨询 ...
目的 构建人miR-373-3p低表达慢病毒载体,探讨miR-373-3p低表达对子宫内膜癌HEC-1A细胞增殖和迁移能力的影响.方法 利用PCR扩增包含miR-373-3p前体序列的目的基因,经酶切后克隆入慢病毒载体GV280中构建GV280-pre-miR-373-3p inhibitor表达载体,通过与包装质粒共转染293T细胞,进行慢病毒的包装和滴度测定.分别用空载...
Using PCR and Western blot, upregulation of miR-373-3p was observed to inhibit EMT progression. Similarly, gain- and loss-of-function studies revealed that ectopic miR-373-3p overexpression inhibited the metastasis of choriocarc...
MiR-373-3p has been shown to play pivotal roles in tumorigenesis and metastasis. However, whether miR-373-3p functions to promote choriocarcinoma metastasis is not clear. The purpose of this study is to determine the function of miR-373-3p in the progression of choriocarcinoma. Methods: In ...
The calculated Area under curve (AUC) levels showed that miR-196a-5p (AUC: 0.64; 95% CI: 0.521–0.759;=0.032) for obstructive CAD and STEMI groups, miR-373-3p (AUC: 708; 95% CI: 0.605–0.812;) for STEMI group and miR-375 (AUC: 0.722; 95% CI: 0.635–0.808;<) for obstructive...
增强miR-146b-3p表达对胰腺癌细胞增殖和凋亡的影响
3p对细胞增殖能力的影响,采用划痕观察miR-373-3p对细胞间接迁移能力的影响,采用Transwell小室实验分析miR-373-3p对细胞直接迁移能力的影响.通过进一步的生物信息学软件预测miR-373-3p的潜在靶基因,并运用Western Blot实验检测上调或下调miR-373-3p的子宫内膜癌HEC-1A细胞中LAST2的表达,初步验证miR-373-3p的作用靶点....
Serum miR-373-3p and miR-194-5p Are Associated with Early Tumor Progression during FOLFIRINOX Treatment in Pancreatic Cancer Patients: A Prospective Multic... Serum miR-373-3p and miR-194-5p Are Associated with Early Tumor Progression during FOLFIRINOX Treatment in Pancreatic Cancer Patients: A ...
Collectively, MSC-AS1 knockdown suppressed cell growth and the chemoresistance of glioma cells to TMZ by regulating miR-373-3p/CPEB4 axis in vitro and in vivo through activating PI3K/Akt pathway. 展开 关键词: Glioma Temozolomide MSC-AS1 miR-373-3p CPEB4 PI3K/Akt ...