Following this, a sequence of molecular biology experiments was conducted to evaluate the impact of miR‐29b‐3p on the biological behaviors of NSCLC cells and their resistance to cisplatin. Additionally, we predicted VEGF was a target gene of miR‐29b‐3p by bioinformatics analysis. We ne...
miR-29b-3p and GRM4 3′-UTR base pairing at a binding site and the sequence of mutant GRM4 3′-UTR (a). RELs of miR-29b-3p in the prefrontal cortex neurons 72 h after lenti-over and lenti-inhib/miR-29b-3p infection (b). RELs of GRM4 protein in the prefrontal cortex neurons...
(B) Schematic of miR-29b-3p and the putative binding sequence at the 3' end of H19 as well as the mutant sequence at the 3′ end of H19 for luciferase assays were shown. (C) Schematic of miR-29b-3p and the putative binding sequence in the 3’-UTR sequence of DNMT3B as well ...
Table 1. RT‒qPCR primer sequences. GenesSequences circ-CARD6 Forward 5′-GCGGCTTAAGGCATGAAGTTT-3′ Reverse 5′-CACTGTGATCTCAGGGGCTT-3′ miR-29b-3p Forward 5′-GCUGGUUUCAAUGGUGCUAGAUA-3′ Reverse 5′-UGACUAAAGUUACCACGAUCUAU-3′ U6 Forward: 5′-CTCGCTTCGGCAGCACA-3′ Reverse: 5...
The sites and sequences of three binding sites were indicated in model scheme. f Binding of c-myc in MCF-7 and MDA-MB-231 cells to the miR-29b-3p promoter region was analyzed by CHIP-qPCR. g qRT-PCR analysis of miR-29b-3p expression in MCF-7 and MDA-MB-231 cells after ...
Here, we isolated a full-length Odorrana tormota MSTN cDNA sequence of 1701bp ( Ot -MSTN), containing a putative N-terminal signal peptide, a TGF-尾 propeptide domain and an active peptide. Ot -MSTN was expressed in 9 selected tissues examined, and the highest level of expression was in...