最后,肝癌样本中免疫组化分析表明,瘤组织中miR-28-5p 与IL-34表达水平显著负相关,与巨噬细胞的浸润、患者总生存期短以及肿瘤患者的复发显著相关。多变量分析显示,miR-28-5p低表达/IL-34过表达或与瘤内巨噬细胞同时存在,都是一个独立的整体生存和癌症复发预后指标(请见图二)。该研究成果创造性地绘制出肝癌细胞...
[结果]miR-28-5p与MTSS1有潜在的结合位点;转染miR-28-5p mimic可明显降低MTSS1-WT的荧光素酶活性,对MTSS1-MUT没有影响,而miR-28-5p inhibitor则表现出相反作用,表明miR-28-5p与MTSS1存在靶向调节作用;A549细胞中miR-28-5p mRNA的相对表达量高于MRC5细胞(P<0.05),A549细胞中MTSS1 mRNA的相对表达量低于MRC...
解决的技术问题:本发明通过筛选心脏抽吸液的microRNA获得miR-28-5p,该microRNAs可用于诊断,缓解和/或治疗心肌梗死。 技术方案:如SEQIDNO.1所示的miR-28-5p在制备治疗心肌梗死药物中的应用。 如SEQIDNO.2所示编码miR-28-5p的基因在制备治疗心肌梗死药物中的应用。 miR-28-5p的mimics在制备治疗心肌梗死药物中的应...
货号:mml-miR-28-5p 原价:￥4030 现价:￥3098.38节省:￥932 规格:1 kits 说明:mml-miR-28-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录产物...
心脏抽吸液miR‑28‑5p在心脏疾病中的应用,属于生从生物学和医学领域,具体涉及通过高通量测序筛选在心梗抽吸液筛选并验证了相应的microRNA,并通过实验验证了此microRNAs在制备诊断、缓解和/或治疗心肌梗死的药物中的应用。 二、法律状态 法律状态公告日法律状态法律状态信息 2019-12-06 实质审查的生效 实质审查的生...
(3)将皮层神经元分为miR-28-5p转染组和无义序列转染组,分别转染miR-28-5p模拟物及其无义对照序列,转染6 h后,均制备H/R诱导的细胞损伤模型。采用RT-qPCR、CCK-8、流式细胞仪分别检测2组神经元miR-28-5p的表达及细胞增殖、凋亡情况;采用ELISA及Western blotting分别检测培养上清中LDH、细胞中MDA、SOD含量和...
Moreover, the LOXL1-AS1 induced regulatory effects on EC cells were partially reversed by miR-28-5p inhibitor. Mechanistically, LOXL1-AS1 competitively bond to miR-28-5p, resulting in upregulation of RAP1B. Additionally, in vivo study confirmed the findings discovered in vitro. Conclusions In ...
miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1 (vol 50, pg 1383, 2017) miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1. Int J Oncol. 2017 Apr 27. https://doi.org/10.3892/... Yao,Shuzhong...
miRNAs were relative to the disease progression of ovarian cancer (3,13–16). The mechanism and function of miR-28-5p have been studied in VHL-associated cancer (17), colorectal cancer (18), and breast cancer (19). However, the functional relevance of miR-28-5p in ovarian cancer is ...
结果病毒感染KFs 48 h后,hsa-miR-199a-5p组的hsa-miR-199a-5p表达量明显高于rLv-NC组和空白对照组,差异有统计学意义(P<0.01);与rLv-NC组和空白对照组相比,hsa-miR-199a-5p组的细胞增殖率、侵袭性均有所降低,细胞凋亡率...