miR-203a-3p转化生长因子-β1(TGF-β1)肝细胞癌目的探索miR-203a-3p对人肝癌细胞系增殖和凋亡的分子调节机制.方法RT-qPCR检测临床肝癌(HCC)组织及肝癌细胞系中miR-203a-3p的表达;建立miR-203a-3p过表达的HepG2细胞模型;MTT法检测细胞增殖;流式细胞测量术检测细胞凋亡;平板集落形成实验检测集落形成;细胞划痕实验...
结论miR-203a-3p可以通过靶向调控VEGF-C表达抑制卵巢癌细胞增殖、侵袭和迁移，其机制可能与抑制增殖相关蛋白和调控上皮间质化过程相关。[关键词]卵巢癌；miR-203a-3p;血管内皮生长因子C;细胞侵袭；细胞迁移 Doi： 10. 3969/j. issn. 1673-5293. 2022. 01. 012 [中图分类号]R737. 31 [文献标识码]A [文章...
构建野生型质粒LASP1-Wt和突变质粒LASP1-Mut后,将其分别与miR-203a-3p mimics、miR-NC mimics共转染至HepG2细胞,记为LASP1-Wt+miR-203a-3p组、LASP1-Wt+miR-NC组、LASP1-Mut+miR-203a-3p组、LASP1-Mut+miR-NC组,细胞培...
此外,rescue实验表明hsa_circ_0043280过表达可以恢复miR-203a-3p降低的PAQR3表达水平,并且减弱miR-203a-3p引起的细胞增殖和迁移能力的增强。这表明hsa_circ_0043280可能作为miRNA海绵介导宫颈癌的肿瘤表型。 图3 Hsa_circ_0043280吸附miR...
荧光素酶报告基因实验证实miR-203a-3p可以直接结合在PRMT53'-UTR上,即PRMT5是miR-203a-3p的直接靶基因;在胃癌组织标本中,PRMT5表达水平显著高于正常组织对照(P〈0.01),并且其表达水平与miR-203a-3p表达呈显著负相关(r=-0.4124〈0.01);过表达miR-203a-3p后,胃癌BGC823细胞的增殖能力显著低于miR-NC对照组(P〈...
货号:dre-miR-203a-3p 原价:￥4030 现价:￥3098.38节省:￥932 规格:1 kits 说明:dre-miR-203a-3p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
结果胶质瘤组织miR-203a-3p水平和阳性表达率显著低于癌旁组织(P<0.05),PHOX2B mRNA水平和阳性表达率显著高于癌旁组织(P<0.05)。Pearson相关性分析,神经胶质瘤患者miR-203a-3p、PHOX2B mRNA水平呈负相关(P<0.05)。miR-203a-3p、PHOX2B与KPS评分、肿瘤组织坏死和WHO分级有关(P<0.05)。Kaplan-Meier曲线分析得出,...
miR-323a-3p regulates lung fibrosis by targeting multiple profibrotic pathways Maladaptive epithelial repair from chronic injury is a common feature in fibrotic diseases, which in turn activates a pathogenic fibroblast response that produces excessive matrix deposition. Dysregulated microRNAs (miRs) can reg...
miR-203a-3p was reported as a tumor suppressor and disregulated in many malignancies including nasopharyngeal carcinoma (NPC). However, its function in tumor growth and metastasis in NPC has rarely been reported. Methods The expression level of miR-203a-3p in human NPC tissues and cell lines wa...
研究组循环miR-203a-3p水平低于对照组;差异具有统计学意义(P<0.05);研究组病理组织SOCS1和SOCS3表达水平低于癌旁组织;病理组织SOCS1和SOCS3甲基化程度高于癌旁组织;差异具有统计学意义(P<0.05);研究组循环miR-203a-3p水平与组织SOCS1和SOCS3表达呈正相关性(r=0.576和r=0.475;P<0.05);与组织SOCS1和SOCS3甲基...