而在骨肉瘤、上皮性卵巢癌中NORAD含量显著升高[30],miR-155-5p表达下降。 另一方面,miR-155-5p在同一类型癌症中出现表达差异可能与不同肿瘤亚型和细胞系有关,如miR-155-5p在肺鳞癌细胞系H2170中高表达并促进其侵袭和迁移[31],然而在肺腺癌A549细胞中,...
The combined treatment with DDP and Andro induced the lowest viability and highest apoptosis in both A549 and A549/DDP cells. MiR-155-5p expression was suppressed, and SIRT was promoted by the Andro treatment, while overexpression of miR-155-5p reversed effects of Andro in cells, which was ...
Stellettin B induces G1 arrest, apoptosis and autophagy in human non-small cell lung cancer A549 cells via blocking PI3K/Akt/mTOR pathway Sci. Rep., 6 (2016), p. 27071 View in ScopusGoogle Scholar Zheng and Wang, 2011 Z.M. Zheng, X. Wang Regulation of cellular miRNA expression by h...
(一)肺癌组织中lncRNA CCT5P2表达上调,较癌旁组织显著增高;肺癌细胞株中lncRNA CCT5P2表达较正常肺上皮明显增高;A549细胞中lncRNA CCT5P2表达主要定位于细胞质.(二)利用shRNA技术成功构建shRNA-CCT5P2肺癌A549和SPC-A-1稳转细胞株;下调lncRNA CCT5P2表达后,A549和SPC-A-1细胞生长,增殖受到抑制;流式细胞术...
Methods and results The antimiR-155-5p and Scramble oligonucleotides were transfected into the A549 human adenocarcinoma lung cancer. After 24 h, the expression level of SIRT1, SIRT2, TP53INP1, and mTOR were evaluated for both groups performing qRT-PCR. After 48 h, the apoptosis assay was ...
There are four types of cells鈥攖hree kinds of animal models and one type of histopathological sections of human lung cancer were utilized as models, including A549, SPC-A1, H446 lung cancer cells, tumor-initiating cells (TICs), subcutaneous and lung xenografts mice, and lox-stop-lox(LSL) ...