另外,当与谷氨酰胺消耗和高铵条件下的miR-105重编程的CAFs共培养时,乳腺癌细胞也显示存活率的增加。为了提供进一步的体内证据,作者将表达miR-105的乳腺癌细胞与表达anti-miR-105或对照序列的CAFs混合,移植到免疫受损的小鼠中。当与表...
近日,复旦大学脑科学研究院来滨和郑平课题组在Translational Psychiatry上发表了题为“Two kinds of transcription factors mediate chronic morphineinduced decrease in miR-105 in medial prefrontal cortex of rats ”的论文,揭示了CREB和GR介导慢性吗啡诱导的大鼠内侧前额叶皮层miR-105的降低。 首先,课题组对盐水组、...
RT-PCR 检测 miR-105 表达水平;成球实验检测细胞成球率、成球直径;Weslern blot 检测 SOX2、OCT4 蛋白表达水平;流式分选检测 CD133+ 细胞数;克隆形成实验检测细胞增殖;Transwell 检测细胞侵袭;Weslern blot 检测 VEGF 蛋白表达水平。结果 癌旁组织中 miR-105 表达水平低于癌组织。与 Control 组相比较,miR-105...
miR-105a-5p靶向调控XAF1，抑制XAF1表达能逆转抑制miR-105a-5p对宫颈癌细胞迁移和侵袭的作用。结论抑制miR-105-5p表达可通过上调XAF1抑制宫颈癌细胞的迁移和侵袭。〔关键词〕miR-105a-5p；XAF1；宫颈癌;迁移;侵袭 〔中图分类号〕R737.33〔文献标识码〕A〔文章编号〕1005-9202(2021)08-1723-05:doi:10....
ppa-miR-105 qPCR试剂盒 分类:microRNA qPCR Kits 品牌:BioTNT 货号:ppa-miR-105 原价:￥4030 现价:￥3098.38 节省:￥932 规格:1 kits 说明:ppa-miR-105 特异性茎环引物试剂盒 ￥615产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因...
结果 宫颈癌细胞HeLa,SiHa中miR-105a-5p的表达水平显著升高;抑制miR-105a-5p表达和过表达XAF1均可抑制宫颈癌细胞迁移和侵袭;抑制基质金属蛋白酶(MMP)-2蛋白的表达,促进E-cadherin蛋白的表达.miR-105a-5p靶向调控XAF1,抑制XAF1表达能逆转抑制miR-105a-5p对宫颈癌细胞迁移和侵袭的作用.结论 抑制miR-105-5p表达...
The MCF2L-AS1/miR-105/IL-1β regulatory axis regulates the resistance of CRC cells to OXA treatment.Keywords: CRC, oxaliplatin, OXA resistance, MCF2L-AS1, miR-105, IL-1βBackgroundColorectal cancer (CRC) is among the most prevalent and deadly cancers in the world, and affected patients of...
(miRNAs) have recently been implicated in the intercellular crosstalk. Here we show a mechanistic model involving breast-cancer-secreted, extracellular-vesicle-encapsulated miR-105, which is induced by the oncoprotein MYC in cancer cells and, in turn, activates MYC signalling in cancer-associated ...
Reduced miR-105-1 levels are associated with poor survival of patients with nonsmall cell lung cancer R, Chang Z, Yang H, et al: Reduced miR‑105‑1 levels are associated with poor survival of patients with non‑small cell lung cancer... G Lu,D Fu,C Jia,... - 《Oncology Lette...
[摘要] 目的 探讨灵芝胶囊对伴(human papillomavirus,HPV)感染宫颈癌患者肿瘤组织 CD105 蛋白及 miR-21 表达的影响。 方法 选取慈溪市中医医院肿瘤科收治的 HPV 阳性的宫颈癌患者患者 162 例,按随机数字表法分组,对照组 81 例予以新辅助化疗, 研究组 81 例在对照组基础上予以灵芝胶囊治疗,检查记录 2 组间肿瘤...