此外,MIR100HG过表达的CC细胞显示E-钙粘蛋白表达减少,间充质基因和EMT-TFs表达增加;相比之下,在MIR100HGKOE4细胞中观察到这些基因的表达增加(图1g和h)。在通过反义寡核苷酸(ASOs)转染MIR100HG过表达或敲除后,SW480(低内源性MIR100HG表达)和SW620(中度内源性MIR100HG表达)细胞中观察到类似的结果(图1i、j)...
基因敲除模型发现,敲除MIR100HG的CC-CR细胞(MIR100HGKOE4)菌落数目在西妥昔单抗药物环境下显著减少,而恢复全长后则消除了对西妥昔单抗的敏感。为了确定上述结果是否可以在体内重现,研究者通过荧光素酶和小鼠皮下注射体内实验发现在MIR100HGKOE4小鼠上荧光信号以及肿瘤体积和重量均显著降低,并且免疫组化(IHC)染色也...
本研究首次揭示了MIR100HG能以非miRNA依赖的方式促进结直肠癌细胞的上皮间质转化(epithelial to mesenchymal transition, EMT), 西妥昔单抗耐药和转移等恶性表型。 其机制是,MIR100HG 与RNA结合蛋白核内不均一核糖核蛋白A2B1(heterogeneous ...
利用RNAscope®荧光原位杂交和免疫组织化学染色技术检测CRC西妥昔单抗治疗前后样本和配对的癌旁、原发灶和淋巴结或远处转移灶组织中MIR100HG,hnRNPA2B1和TCF7L2的表达,发现MIR100HG, hnRNPA2B1和TCF7L2在西妥昔单抗治疗后相较于治疗前显著升高,在转移灶中的表达也明显升高,并且MIR100HG和hnRNPA2B1与TCF7L2的...
近日,重庆医科大学王健宇/邢若曦以及重庆市妇幼保健院(重庆医科大学附属妇女儿童医院)李竞宇团队合作在国际学术期刊Journal of Nanobiotechnology上发表了题为Exosomal lncRNA Mir100hg from Lung Cancer Stem Cells Activates H3K14 Lactylation to Enhanc...
In conclusion, we demonstrated the aberrant upregulation of lncRNA MIR100HG in BLM-caused lung fibrogenesis and TGF-β1-stimulated MLE 12 cells. The MIR100HG/miR-29a-3p/Tab1 axis could modulate TGF-β1-induced fibrotic changes in type II alveolar epithelial cells and, thus, might be ...
Fig. 1:MIR100HGis induced by TGFβ. aSchematic outline of the experimental design to identify TGFβ-regulated lncRNAs.bTotal numbers of lncRNA genes regulated by TGFβ1 in a time-course experiment, identified by microarray analysis.c–fReal-time RT-qPCR for determination ofMIR100HGexpression ...
MIR100HG promotes proliferation in TNBC cells Fig. 1: Upregulation of MIR100HG increased TNBC cell proliferation. Full size image Fig. 2: Downregulation of MIR100HG decreased TNBC cell proliferation. Full size image MIR100HG leads to cell cycle arrest at G1/S via p27 regulation ...
Here, we identified lncRNA MIR100HG as a pro-oncogene for TNBC progression. Knockdown of MIR100HG decreased cell proliferation and induced cell arrest in the G1 phase, whereas overexpression of MIR100HG significantly increased cell proliferation. Furthermore, MIR100HG regulated the p27 gene to ...
2)通过QPCR验证了MIR100HG和miR-100、miR-125b在CC-CR中高表达,同时利用TCGA数据库分析了MIT100HG的表达和miR-100、miR-125b的相关性,发现他们的确是有紧密联系。作者还利用FISH发现与CC异种异种移植瘤相比MIR100HG和miR-100、miR-125b在CC-CR异种移植瘤中富集。并分析其他30种CRC细胞系中MIR100HG和miR-100...