在老年小鼠膝关节OA模型中,miR-29b-5p的持续过度表达导致软骨增厚,MMP13和P21(细胞周期蛋白依赖性激酶抑制剂1A)下调。软骨细胞中的荧光原位杂交(FISH)显示miR-29b-5p主要定位于细胞质中。这些结果表明,miR-29b-5p是细胞质中的一种保护性miRNA,通过过度表达诱导分解代谢酶和衰老相关基因的下降以及软骨ECM合成的增加...
miR-29b-2-5pNFATc1cardiac hypertrophyPathological cardiac hypertrophy is one of the notable causes of heart failure. Circular RNAs (circRNAs) have been studied in association with cardiac hypertrophy; however, the mechanisms by which circRNAs regulate cardiac hypertrophy remain unclear. In this study,...
使用反义寡核苷酸抑制miR-181a-5p可减轻小关节软骨退变的严重程度。包括microRNAs在内的非编码RNAs在软骨细胞衰老中起重要作用。据报道,MiR-29b-5p可缓解软骨细胞衰老,p21和p16表达降低。然而,机械超负荷是否通过microRNAs调节软骨细胞衰...
(A&B)WB和qRT-PCR结果:用agomir-29b-5p、agomir-NC、antagomir-29b-5p、或antagomir-NC转染大鼠软骨细胞2天后,利用WB(A)和qRT-PCR(B)实验验证COL2A1、aggrecan、SOX9、MMP3、MMP13、ADAMTS4、ADAMTS5、P16INK4a和P21的表达...
小关节退变伴有microRNA表达的改变。使用反义寡核苷酸抑制miR-181a-5p可减轻小关节软骨退变的严重程度。包括microRNAs在内的非编码RNAs在软骨细胞衰老中起重要作用。据报道,MiR-29b-5p可缓解软骨细胞衰老,p21和p16表达降低。然而,机械超负荷是否通过microRNAs调节软骨细胞衰老仍然未知。
货号:cgr-miR-29b-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:cgr-miR-29b-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
2 蛋白表达水平显著降低(P<0.05),miR-29b mimics+参芪扶正注射液组 Bcl-2 蛋白表达水平进一步降低(P<0.05); miR-29b inhibitors 组 Patu8988/5-Fu 细胞 Bcl-2 蛋白表达水平显著升高(P<0.05),miR-29b mimics+参芪扶正注射液组 Bcl-2 蛋白水平无明显变化.结论 miR-29b 通过下调 Bcl-2 蛋白表达抑制 Pat...
由miR-29前体的3p臂剪切修饰产物(miR-29或miR-29-3p)是miR-29家族的主要成员,而5p臂形成的家族成员(miR-29*或miR-29-5p)也是客观存在的。因此,人类miRNA-29家族有3个主要成员包括hsa-miR-29a、hsa-miR-29b和hsa-miR-29c [ 1 ]( 图1 )。3个miR-29家族成员主要分布在2个染色体上:miR-29b2/miR-29...
As shown in Figure 1a, comparing both data sets, just three miRNAs, namely miR-574-5p, miR-29b and miR-193, were upregulated in the presence of maturation stimuli (GSE36316), whereas, on the other hand, were downregulated in the presence of cancer cells (GSE42722). We then evaluated ...
As shown in Figure 1a, comparing both data sets, just three miRNAs, namely miR-574-5p, miR-29b and miR-193, were upregulated in the presence of maturation stimuli (GSE36316), whereas, on the other hand, were downregulated in the presence of cancer cells (GSE42722). We then evaluated ...