在Science期刊中名为Stem cell–homing hydrogel-based miR-29b-5p delivery promotes cartilage regeneration by suppressing senescence in an osteoarthritis rat model的论文证实,一种miRNA(miR-29b-5p)能抑制软骨细胞的衰老和合成代谢/分解代谢失衡,从而抑制下游的10-11易位酶1(TET1)基因,并且在前交叉韧带横断(ACLT...
图1.确认miR-29b-5p的功能和靶基因。 (A&B)WB和qRT-PCR结果:用agomir-29b-5p、agomir-NC、antagomir-29b-5p、或antagomir-NC转染大鼠软骨细胞2天后,利用WB(A)和qRT-PCR(B)实验验证COL2A1、aggrecan、SOX9、MMP3、MMP13、A...
Overall, this study uncovers a novel NRF2/miR‐29b‐1‐5p/AKT regulatory loop that can regulate the fate (life/death) of MDA‐MB‐231 cells and suggests this loop as therapeutic target for TNBC.doi:10.1002/jcp.29062AnnaDepartmentDeDepartment...
据报道,MiR-29b-5p可缓解软骨细胞衰老,p21和p16表达降低。然而,机械超负荷是否通过microRNAs调节软骨细胞衰老仍然未知。 在湖南省中南大学湘雅医院脊柱外科、器官损伤衰老与再生医学湖南省重点实验室等研究团队一项研究中,证明了机械超负荷...
一种与衰老相关的miRNA, miR-29b-5p,首次在OA软骨中被发现显著下调,其上调通过10 - 11易位酶1 (TET1)抑制基质金属蛋白酶和衰老相关基因(P16INK4a/P21)的表达。一种可注射的生物活性自组装肽纳米纤维水凝胶被应用于递送agomir-29b-5p,该agomir-29b-5p是通过偶联干细胞归位肽SKPPGTSS实现功能化的,同时用于内...
小关节退变伴有microRNA表达的改变。使用反义寡核苷酸抑制miR-181a-5p可减轻小关节软骨退变的严重程度。包括microRNAs在内的非编码RNAs在软骨细胞衰老中起重要作用。据报道,MiR-29b-5p可缓解软骨细胞衰老,p21和p16表达降低。然而,机械超负荷是否通过microRNAs调节软骨细胞衰老仍然未知。
We have identified one of these as the passenger strand of the key osteogenic microRNA-29b-1 (miR-29b-1-5p). This novel passenger strand increases with age in human and murine BMSCs (Hill et al., 2016; Periyasamy-Thandavan et al., 2013; Baglio et al., 2013; Lee et al., 2016;...
货号:chi-miR-29b-5p 原价:￥4030 现价:￥3098.38节省:￥931.62 规格:1 kits 说明:chi-miR-29b-5p 特异性茎环引物试剂盒 ￥615 产品参数 ---说明书下载--- microRNAs(miRNAs)是一类长度约19~24nt的非编码单链RNA,通过碱基互补配对的方式与靶基因的29’UTR区部分或完全互补,剪切靶基因的转录产物或者抑制转录...
29b 模拟物(miR-29b mimics)和 miR-29b 抑制剂(miR-29b inhibitors)转染至人胰腺癌 5-氟尿嘧啶(5-fluorouracil,5-Fu)耐药细胞株 Patu8988/5-Fu,采用 qRT-PCR 检测参芪扶正注射液(5,10,20 μL/mL)对 Patu8988/5-Fu 细胞 miR-29b mRNA 表达的影响;采用 MTT 法检测 miR-29b mimics 和 miR-29b ...
As shown in Figure 1a, comparing both data sets, just three miRNAs, namely miR-574-5p, miR-29b and miR-193, were upregulated in the presence of maturation stimuli (GSE36316), whereas, on the other hand, were downregulated in the presence of cancer cells (GSE42722). We then evaluated ...