中文名称:MIO-M1 Cell|视网膜Muller干细胞 英文名称:MIO-M1 Cell 保存条件: 常温培养或液氮冻存 纯度规格: MIO-M1 Cell|视网膜Muller干细胞 产品类别: 生物试剂 "MIO-M1 Cell|视网膜Muller干细胞 细胞背景资料:视网膜Muller细胞;自发永生;女性 细胞形态:上皮细胞样 体内细胞培养及其操作步骤:【瘤细胞悬液接种】1...
中文名称:MIO-M1|视网膜Muller干细胞英文名称:MIO-M1 Cell 保存条件:低温避光纯度规格:1×10(6)cells/T25培养瓶 产品类别:实验试剂 "MIO-M1|视网膜Muller干细胞 细胞背景资料:视网膜Muller细胞;自发永生;女性 细胞形态特性:详见细胞说明书 悬浮细胞不容易转染:悬浮细胞是指细胞生长不依赖支持物表面,在培养液中呈悬...
MIO-M1 cells and similar muller glial cell lines derived from adult human retina exhibit neural stem cell characteristics 1. Stem Cells 25, 2033e2043.Lawrence JM, Singhal S, Bhatia B, Keegan DJ, Reh TA, Luthert PJ, Khaw PT, Limb GA (2007) MIO-M1 cells and similar muller glial cell ...
产品名称: MIO-M1,视网膜Muller干细胞 来源ATCC,DSMZ,Sciencell MIO-M1,视网膜Muller干细胞 上皮样细胞呈多角形,尺寸更为规则,贴附在基质上呈散在斑片状生长。 MIO-M1,视网膜Muller干细胞 培养实验室的具体要求主要
Cell cryopreservation 1.冻存液:92%*培养基+8%DMSO(可以根据实验室条件自行选择) 2.降温步骤:4度10min,-20度2h,-80过夜后液氮保存。 冻存方法: 1.消化并离心获得细胞沉淀后,加配置好的冻存液轻轻重悬细胞;2.将细胞悬液尽快移入已经做好标记的冻存管;3.将冻存管转入程序冻存盒,放入-80度冰箱过夜,第二...
We first reported the M眉ller glial characteristics of the spontaneously immortalized human cell line MIO-M1, but recently we have derived similar cell lines from the neural retina of several adult eye donors. Since immortalization is one of the main properties of stem cells, we investigated ...
MIO-M1 cell lineMüller cellsLactateGlutamateUsing the human M眉ller cell line, MIO-M1, the aim was to study the impact of mitochondrial inhibition in M眉ller glia through antimycin A treatment. MIO-M1 cell survival, levels of released lactate, mitochondrial function, and glutamate uptake were ...
Treatment with bevacizumab did not reduce MIO-M1 cell viability, but increased metabolic activity at 24 h (0.5 mg/mL) and induced apoptosis and autophagy, as shown by the increased caspase-3 levels at 12 h (0.25 and 0.5 mg/mL) and the increased beclin levels at 24 h (0...
As the RAAS is hypothesized to play an important role in the pathogenesis of diseases that threaten vision, such as diabetic macular edema or retinal vein occlusion, the possible changes induced by exposure of the human cell line MIO-M1, an established model of Mueller cells, to an...
Cell viability increased with estradiol 20nM in ARPE-19 (p=0.008) and MIO-M1 (p<0.0001) CoCl2-treated cells; Estradiol 40nM increased CV (p<0.0001) and MitoMP (p=0.015) in CoCl2-treated MIO-M1 cells. CoCl2-treated ARPE-19 cells showed increased MitoMP in 1x and 2x brimonidine, p=...