Wild-type males unfamiliar with the test mice were used as stimulators, and their tail tips were painted black with a marker for identification before habituation. An age-matched pair of test and stimulator mice was placed in a non-transparent acrylic open arena (50 Â 50 Â 40 cm) ...
Co-localization of p62 and LC3B (yellow) can therefore be used as a marker of a utophagosomes22. The staining with p62 and LC3B revealed the presence of the same large vesicles as were seen in the electron microscopy images, here seen as empty black circles (Fig. 5a). Overall, ...
We hypothesized that cancer cachexia involves systemic acceleration of autophagy. For autophagy to be accelerated systemically, autophagy-inducing bioactivity must be present in circulation. Sera from 79 patients from The Central Norway Lung Cancer Biobank (CNLCB), collected at the time of diagnosis, a...
Gfap-positive astrocytes and Iba1-positive microglia.n=98,890–145,032 cells per coverslip counted. Values are mean±s.e.m. of four biological replicates. Scale bar, 100 μm. (c) Cortical cultures exhibit expression of marker genes representative of nine cell types found in the brain (ya...
TCRm antibodies/TCRm-ADCs pre-incubated with K562-A2-WT1126–134 at 37 ◦C were stained with Cy5-labeled anti-human Fc antibody and lysosomal-associated membrane protein-1 (LAMP-1, the lysosomal marker) was stained by Cy3. As shown in Figure 3B, we found that both TCRm antibodies and...
Lung sections were also stained with Masson's Trichrome stain for the identification of fibrosis, and by immunohistochemistry using the human alveolar macrophage marker, HAM56 (Dako, Carpinteria, CA, USA). Histologic examination of the lungs was done by an experienced veterinary pathologist (DLP)...
We previously reported that PARP inhibition could hinder the potential of melanoma cells for VM formation on Matrigel [14]. Moreover, PARP inhibition markedly reduced the expression of VE-cadherin, a well-known marker of VM [13], as well as the phosphorylation of VE-cadherin on their use to...