The developing organoids stained positive for a marker (SOX1) of nerve cell generation. As development continued, less cells showed markers of generation and more cells showed markers of spinal motor neuron maturation (ISL1). Credit: Reproduced from Ref. 1 and licensed under CC BY 4.0 © 20...
Co-localization of p62 and LC3B (yellow) can therefore be used as a marker of a utophagosomes22. The staining with p62 and LC3B revealed the presence of the same large vesicles as were seen in the electron microscopy images, here seen as empty black circles (Fig. 5a). Overall, ...
Wild-type males unfamiliar with the test mice were used as stimulators, and their tail tips were painted black with a marker for identification before habituation. An age-matched pair of test and stimulator mice was placed in a non-transparent acrylic open arena (50 Â 50 Â 40 cm) ...
The genetic study did not find any DFT-related mutations, includingAPP,CHMP2B,FUS,GRN,HNRNPA1,HNRNPA2B1,ITM2B,MAPT,PRNP,PSEN1,SNCA,SNCB,TARDBP,TIMM8A,TREM2,UBQLN2, andVCP[21]. The result with respect toC9orf72in this patient was negative regarding RP-PCR expansion. Noticeably, the genet...
Lung sections were also stained with Masson's Trichrome stain for the identification of fibrosis, and by immunohistochemistry using the human alveolar macrophage marker, HAM56 (Dako, Carpinteria, CA, USA). Histologic examination of the lungs was done by an experienced veterinary pathologist (DLP)...