microscope equipped with a Flexacam c5 digital camera. The possible range of magnification values, minimum to maximum, for the discussed UHD or FHD monitor sizes (Table 2) and pixel ratios (Table 3, N.B.: 2x2 camera-pixel binning required for the 21.5” FHD monitor, refer to figure 2)....
She also asks people to consider potential decisions before preparing samples, in particular, whether correlation with light microscopy will be required (e.g., for technical orientation or targeting studies). Early considerations such as these influence how you prepare your samples. “Consider only th...
tumor processes, storage disorders, and infectious agents. This method provides numerous advantages such as high magnification (×10,000 and high resolution of 0.3nm), individual particle examination, and particle shape measurement. However, this method is expensive and a trained operator is required....
The optical microscope has revolutionized biology since at least the 17th Century. Since then, it has progressed from a largely observational tool to a powerful bioanalytical platform. However, realizing its full potential to study live specimens is hind
We show the cost of insisting on verification by positive result in search algorithms; for the zero-half-lie case bisectioning with verification incurs a 50% penalty in the average number of queries required. The optimal partitioning of search spaces directly following verification in the presence ...
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When used appropriately, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. The confocal microscope’s ability to block out-of-focus light and thereby perform optical sectioning through a sp
At the Maastricht University and Synapse spin-off fundamental research is taking place and practical detection methods are being developed for thrombosis and bleeding. A good snapshot of the clotting helps researchers to gain a better grasp of the phenomenon. The case study addresses the use of ...
To analyze the super-speed property of our super-resolution endo-microscopy, we address the following questions: how many measurements are actually required to resolve the sub-diffraction features of our sample and how are the imaging speed and the resolution related to each other? In our simulati...
(Fig.7a). While saturated SIM can theoretically yield an infinite resolution, in practice, its resolution is limited by factors such as the SNR and photostability “soft” materials. In addition, extremely high light intensity is required to saturate SIM, which can accelerate photobleaching and ...