This provides the flexibility often required for AFM systems used in multi-user facilities or multi-focused research groups. Investigators who are mainly focused on biological research and/or AFM integration with light microscopy techniques should refer to Bruker’s BioAFM systems to best address ...
microscope equipped with a Flexacam c5 digital camera. The possible range of magnification values, minimum to maximum, for the discussed UHD or FHD monitor sizes (Table 2) and pixel ratios (Table 3, N.B.: 2x2 camera-pixel binning required for the 21.5” FHD monitor, refer to figure 2)....
While light microscopy detects decay at very early stages, it does not appear to be practical for field use and extensive training is generally required for this time-consuming procedure. Light microscopy can be useful along with culturing to identify the features of decay that can be difficult ...
and also to fully characterise the intensity changes required by the varying PSF widths. Finally, before such an approach is applied to biological systems, attempts should be made to include penalty functions characterising photon exposure and/or damage, as well as accounting for practical consideratio...
The optical microscope has revolutionized biology since at least the 17th Century. Since then, it has progressed from a largely observational tool to a powerful bioanalytical platform. However, realizing its full potential to study live specimens is hind
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the viewer maps the two-dimensional image coordinates at sub-pixel resolution into three-dimensional slide coordinates at millimeter resolution as required by SCOORD3D graphic types. To this end, the viewer constructs an affine transformation matrix from image metadata using equations defined in the st...
Robust segmentation required four classes: 1. Background outside cell, 2. Background inside cell, 3. Fine filaments (actin cortex) and 4. Bright patches (focal adhesions). After training, we exported probability maps for class 3 (actin cortex) as 16-bit time-lapse stacks. Then, we ...
To analyze the super-speed property of our super-resolution endo-microscopy, we address the following questions: how many measurements are actually required to resolve the sub-diffraction features of our sample and how are the imaging speed and the resolution related to each other? In our simulati...
When used appropriately, a confocal fluorescence microscope is an excellent tool for making quantitative measurements in cells and tissues. The confocal microscope’s ability to block out-of-focus light and thereby perform optical sectioning through a sp