MINSTED quantifies tiny movements of individual biomolecules with high spatiotemporal precision to successfully resolve the steps of the molecular motor protein kinesin-1 labeled with a single fluorophore as it switches protofilaments. Lukas Scheiderer , Henrik von der Emde & Stefan W. Hell Article...
“The most important thing to consider is always the final objective. What does one want to achieve in the end?” She also asks people to consider potential decisions before preparing samples, in particular, whether correlation with light microscopy will be required (e.g., for technical orientat...
the times required to collect 16 localizations (1 nm RESI precision givenσDNA-PAINT = 4 nm) vary between 42 s (R2, 800 pM, 100 ms exposure time) and 314 min (R5, 50 pM, 200 ms exposure time).
Fluorescence was ignored for several reasons, including its little practical value for rendering natural scenes, and the absence of convenient spectral rendering frameworks that could handle fluorescence efficiently [24, 25]. Glassner presented the first steps towards a correct formulation of the ...
To avoid this complication, the MTF of the K2 Summit was measured with a very low dose rate (~1 e/pixel/s) that required using long exposures (~300 s). 3. Results The physical properties of the three detectors are summarised in Table 1. The high sensitivity of MAPS detectors to ...
The pull-off force is the least required force to separate the contacted probe tip and sample surface. It is found that the pull-off force on Si-face of 6H-SiC crystal is about twice as large as that on C-face, if the two-faces of a SiC crystal are treated with the same ...
Therefore, the autofocus system becomes indispensable, especially for long-term manufacturing and large-scale fabrication in practical applications. To overcome the defocus of the substrate caused by a variety of factors (e.g., thermal drift, mechanical vibrations, and the tilt of the substrate ...
The focus of the present work was placed on the three-dimensional particles and capsules with rather thin polyelectrolyte layers due to their practical relevance (e.g., for the encapsulation of proteins), which required alternative analytical tools. Thus, whispering gallery mode (WGM) experiments ...
Second, it is essential when considering an Open- SPIM to factor in the hidden costs involved, most obvi- ously the costs implied by the time spent building the microscope, learning to use it and learning to use the open source software required to run the microscope and to process the ...
Unfortunately, applications in biology were hampered due by the high peak intensities required for priming 2PE fluorescence. This obstacle was overcome with the advent of ultrashort and fast pulsed lasers in the 80s [19]. Denk and colleagues in a seminal paper on 2PE laser scanning fluorescence...