Fast-adaptive super-resolution lattice light-sheet microscopy for rapid, long-term, near-isotropic subcellular imaging Meta-learning-empowered reflective lattice light-sheet virtual structured illumination microscopy (Meta-rLLS-VSIM) offers near-isotropic resolution for fast and versatile super-resolution ...
Perform the following steps for all sampled objects in a batched fashion: a. Sample a random point from the object, which is used as a positive input point, or use the bounding box of the object as the prompt. b. Predict the mask and expected IOU value for the given input with SAM....
We also demonstrate the practical effects of small misalignments to illustrate both the optimized signal quality and the degree of accuracy required to achieve it. We hope that this guide increases the accessibility of this type of instrumentation and helps researchers use it to produce data of the...
It is typically required to determine the location and frequency of occurrence of these objects within the volume data set. There are three steps involved in performing a spatial distribution analysis: (1) identify objects, (2) determine their local position relative to the 3D imaged volume, and...
(fluorophores) that will be used for the actual biological sample. However, because of the differences in the immediate fluorophore environment, the sample of interest might still perform different from the model samples, and thus further optimization might be required. Fluorescent beads are less ...
While epi-fluorescence microscopy provides the high degree of time resolution which is required to visualise fast processes, confocal laser scanning microscopy (CLSM) offers several advantages over conventional optical microscopy, including controlled depth of field, the elimination of image degrading out-...
Second, it is essential when considering an Open- SPIM to factor in the hidden costs involved, most obvi- ously the costs implied by the time spent building the microscope, learning to use it and learning to use the open source software required to run the microscope and to process the ...
required, Figure 8 MPE simplified optical schemes.Descanned (red dot) and non-descanned (blue dot) schemes for 2PE microscopy. Due to the confinement of the excitation process when operating in non-descanned mode the collection efficiency in depth is increased as shown by the two side views ...
Though the exact reason has not been thoroughly explored, it has been suggested that the high energy light required to excite probes in this region, combined with the high intensities generally required to induce switching, quickly result in greater degrees of photo-damage compared to excitation of...
The disadvantage of this method is that the enzymes required are impermeable to plasma membranes, limiting their applications to surface proteins or fixed cells. Alternatively, simpler systems based on the His-tag and FlAsH-tag employ small-molecule ligands interacting with short poly-histidine or ...