MINSTED quantifies tiny movements of individual biomolecules with high spatiotemporal precision to successfully resolve the steps of the molecular motor protein kinesin-1 labeled with a single fluorophore as it switches protofilaments. Lukas Scheiderer , Henrik von der Emde & Stefan W. Hell Article...
the times required to collect 16 localizations (1 nm RESI precision givenσDNA-PAINT = 4 nm) vary between 42 s (R2, 800 pM, 100 ms exposure time) and 314 min (R5, 50 pM, 200 ms exposure time).
easy to optimize using simple momentum-based stochastic gradient descent optimizers like stochastic gradient descent. Such first-order optimizers sacrifice performance and efficiency for computational simplicity. When optimization speed becomes critical, more advanced optimizers are required. One approach is to...
Fluorescence was ignored for several reasons, including its little practical value for rendering natural scenes, and the absence of convenient spectral rendering frameworks that could handle fluorescence efficiently [24, 25]. Glassner presented the first steps towards a correct formulation of the ...
To avoid this complication, the MTF of the K2 Summit was measured with a very low dose rate (~1 e/pixel/s) that required using long exposures (~300 s). 3. Results The physical properties of the three detectors are summarised in Table 1. The high sensitivity of MAPS detectors to ...
The pull-off force is the least required force to separate the contacted probe tip and sample surface. It is found that the pull-off force on Si-face of 6H-SiC crystal is about twice as large as that on C-face, if the two-faces of a SiC crystal are treated with the same ...
Therefore, the autofocus system becomes indispensable, especially for long-term manufacturing and large-scale fabrication in practical applications. To overcome the defocus of the substrate caused by a variety of factors (e.g., thermal drift, mechanical vibrations, and the tilt of the substrate ...
(nano) materialsFungal and Yeast Cell Wall Polysaccharides—Bioactivities and Practical ApplicationsLatest Research on Polysaccharides: Structure and ApplicationsLignocellulose: Properties, Characterization and ApplicationsMicrobial Polysaccharides as Molecules with Tremendous Biological ImportanceNew Insights into ...
Unfortunately, applications in biology were hampered due by the high peak intensities required for priming 2PE fluorescence. This obstacle was overcome with the advent of ultrashort and fast pulsed lasers in the 80s [19]. Denk and colleagues in a seminal paper on 2PE laser scanning fluorescence...
If the blood sample is diagnosed as positive (i.e. parasites present) an additional capability of differentiating species and life-stages (i.e. identification) is required to specify the infection. From the computer vision point of view, diagnosis of malaria is a multi-part problem. A ...