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mitochondrial network of skeletal muscles that are reflective of muscle metabolism and myogenesis; and patterns of collagen fibers in the bone that are reflective of the organization and health of bone extracellular matrix.Results: We present an analysis of mouse skeletal muscle in healthy and injured...
AM-FM Texture Segmentation in Electron Microscopic Muscle Imaging.Focuses on a study which described the application of an amplitude modulation-frequency modulation (AM-FM) image representation in segmenting electron micrographs of skeletal muscles. Details on the AM-FM method; Results of AM-FM ...
Full size image In these all cells and tissues, the roughness of the image quality was unnoticeable, even at a high magnification of approximately 20,000×. Furthermore, good staining results have also been obtained with insect flight muscle embedded in Araldite-506 epoxy resin as another example...
Full size image Figure 3 Double immunostaining of human testis sections. (A,B) Representative photomicrographs of immunofluorescence labeling for CD34 (green) and α-smooth muscle actin (α-SMA; red). Nuclei are counterstained in blue with 4′,6-diamidino-2-phenylindole (DAPI). CD34-negative/...
L Duchen - 《Journal of the Neurological Sciences》 被引量: 90发表: 1974年 AM-FM texture segmentation in electron microscopic muscle imaging This paper describes the application of an amplitude modulation-frequency modulation (AM-FM) image representation in segmenting electron micrographs of ske... ...
Filopodia, retraction fibers and microvilli, are fragile microextensions of the plasma membrane that are easily damaged by mechanical force during specimen preparation for microscopy. To preserve these structures for electron microscopy glutaraldehyde is generally used, but it often causes antigen masking....
Electron microscopic examination revealed abnormal accumulations of mitochondria in the skeletal muscle, smooth muscle and cardiac muscle. The activity of ... Y Ihara,R Namba,S Kuroda,... - 《Journal of the Neurological Sciences》 被引量: 316发表: 1989年 ...
Full size image We generated different DG constructs characterized by a myc-tagged α-subunit and the C-terminus of the β-subunit fused to the intrinsic fluorescent protein GFP. The insertion of the myc tag was rationally based on the 3D structure of the N-terminal domain of α-DG [10];...
can be increased 100-fold—more than 400 cells have been trapped simultaneously70. The number is limited by the irradiance and damage threshold of the optics and AODs. Thus, hundreds of cells could be manipulated into position concomitantly under computer-control using image recognition software to...