secretion.Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims.Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis,which would be summarized in this mini-review....
In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advan- tages, including low cost, low sample volumes, high throughput and precision. This review ...
There is still ample room for the development of microfluidics to deal with the severe, ongoing pandemic. More significantly, new infectious diseases may emerge in the near future. The traditional technologies are mature and optimized, but require multiple steps and frequent transfers of samples betwe...
These sensors are becoming increasingly versatile, able to detect multiple analytes simultaneously and to be integrated with other technologies such as microfluidic pumps and valves. As a result, these sensors have the potential to be used in a wide range of healthcare applications, from disease dia...
The advantages and limitations of these technologies are summarized and challenges to establish the integrated microfluidic biosensing systems for real-time single-cell secretomics are discussed. Recent efforts on integrated platforms for on-chip single-cell protein assays are highlighted and some ...
Infectious diseases are posing problems for public medical systems and attracted much attention from the public and scientists. Microfluidics is one of the best technologies to conduct molecular diagnostics for infectious diseases and has made great achievements, especially during the outbreak of COVID-19...
Functionalized cassettes included an internal negative control zone, spotted with no antigen, an internal positive control zone, spotted with 20 μg mL−1 rabbit anti-goat IgG antibody (Life Technologies) and multiplexed com- bination of three target antigen zones. All zones were treated ...
We provide a critical evaluation of how droplet-based microfluidic systems should be manufactured and the importance of integrating appropriate detection technologies to probe the small analytical volumes that are representatives of the technology set. We then discuss issues related to data collection and...
The application of microfluidics technologies to the study of retinal function and response holds great promise for development of new and improved treatments for patients with degenerative retinal diseases. Restoration of vision via retinal transplantation therapy has been severely limited by the low ...
One of the great challenges in science and engineering today is to develop technologies to improve the health of people in the poorest regions of the world. Here we integrated new procedures for manufacturing, fluid handling and signal detection in microfluidics into a single, easy-to-use point-...