secretion.Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims.Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis,which would be summarized in this mini-review....
In the past few years, microfluidics-based technologies have gradually emerged and have come to play an essential role in the isolation, detection and analysis of EVs. Such technologies have several advan- tages, including low cost, low sample volumes, high throughput and precision. This review ...
Microfluidics-based technologies are an attractive platform for the detection of infectious pathogens because they offer short processing times, reduced sample consumption, and a miniaturized format with portability and simplicity. Moreover, the integration of multiple functions into a single unit with full...
90]. Traditional CRISPR-based methods are usually time-consuming and labor-intensive because of multiple procedures encompassing nucleic acid extraction, amplification, and CRISPR detection. The likelihood of false-positive results may be increased for exposing liquid to air. Given the above, the CRISPR...
including but not limited to LAMP, recombinase polymerase amplification (RPA), and nucleic acid sequence-based amplification [65,66,67,68]. With these technologies, nucleic acids are amplified at a constant temperature, thus promoting portable POCT devices for molecular diagnostics with low cost and...
Use of microfluidics-based technologies enable hundreds of thousands of microdroplets to be generated at a lower cost. Surrounded by oil, these aqueous droplets have a volume equivalent to cell size and contain a bead with a cell-specific barcode and a single cell. At present, the most popular...
Microfluidics technology is revolutionizing various industries, particularly in life sciences and medical research. Integrated microfluidic components fabricated using techniques like soft lithography are transforming drug development and delivery technologies. These systems enable precise handling of small volumes ...
Here, recent advances in microfluidics-based single-cell manipulation and emerging approaches for label-free single-cell biosensing are reviewed. The advantages and limitations of these technologies are summarized and challenges to establish the integrated microfluidic biosensing systems for real-time single...
A model microfluidics-based system for the human and mouse retinaRetinaProgenitorSDF-1DiffusionMigrationThe application of microfluidics technologies to the study of retinal function and response holds great promise for development of new and improved treatments for patients with degenerative retinal diseases...
foodborne pathogens. Microfluidic technology has opened the research gateway for developing low-cost, on-site, portable, and rapid assay devices. The article includes the application of microfluidic-based devices to identify critical food pathogens and briefly discusses the necessary research in this ...